Neural Regeneration Research

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Lycium barbarum polysaccharides protected retinal ganglion cells against oxidative stress injury

Lian Liu 1 , Xiao-Yuan Sha 1 , Yi-Ning Wu 2 , Meng-Ting Chen 1 , Jing-Xiang Zhong 1   

  1. 1 Department of Ophthalmology, Affiliated First Hospital of Jinan University, Guangzhou, Guangdong Province, China
    2 Department of Ophthalmology, Guangdong Women and Children Hospital,Guangzhou 511400, China.)
  • Online:2019-12-19
  • Contact: Jing-Xiang Zhong, Ph.D., zjx85221206@126.com.

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Abstract:

AIM: To investigate the protective effect and its mechanism of Lycium barbarum polysaccharides (LBP) against oxidative stress injury in retinal ganglion cells-5 (RGC-5).
METHODS: RGC-5 cells were exposed to different concentrations of CoCl 2 for 24 hours, then cell viability was measured to get the properly concentration of CoCl 2 which can induce half apoptosis of RGC-5. With different concentrations of LBP pretreatment, the RGC-5 cells were then exposed to appropriate concentration of CoCl 2 , cell apoptosis was detected by flow cytometric analysis. ROS and mitochondrial membrane potential(MMP) were test by the kits.
RSULTS: LBP could effectively reduce the CoCl 2 -induced RGC-5 cells’ apoptosis. LBP inhibited ROS generation and the reduction of the mitochondrial membrane potential(MMP).
CONCLUSION: LBP could protect RGC-5 cells from CoCl 2 -induced apoptosis. MMP and ROS had relationship with the protective effects of LBP.

Key words: Retinal ganglion cells, Lycium barbarum polysaccharides, oxidative stress injury, Cell apoptosis