神经退行性病

    Phosphoinositide-3-kinase regulatory subunit 4 participates in the occurrence and development of amyotrophic lateral sclerosis by regulating autophagy
  • Figure 3|Distribution of PIK3R4-positive cells in the anterior horn, posterior horn, and areas within and around the central canal in the cervical, thoracic, and lumbar regions of the spinal cord in WT and TG mice (immunofluorescence staining).

    Immunofluorescence staining demonstrated that PIK3R4-positive cells were extensively distributed in the anterior horn, central canal, the area surrounding the central canal, and the posterior horn of the cervical, thoracic, and lumbar segments of the spinal cord during the pre-onset, onset, and progression stages in both the WT and TG mice (Figure 3). PIK3R4-positive cells decreased in the anterior horn of the cervical, thoracic, and lumbar cord segments of TG mice during progression (Figure 3). Based on “rough” observations using fluorescence microscopy, the general tendency was decreased PIK3R4 distribution in the spinal cord of TG mice at pre-onset, onset, and progression stages compared with WT mice. However, a detailed fluorescence data analysis was not performed.


    Figure 4|PIK3R4 and NeuN double-positive cells in the AH, PH, and areas around the CC of the spinal lumbar segment at different stages in both WT and TG mice (immunofluorescence staining). 

    Immunofluorescence staining showed that PIK3R4 was extensively expressed and distributed within NeuN-positive neuronal cells in the anterior horn, posterior horn, and central canal of the spinal lumbar segments. PIK3R4 expression and distribution in neurons of the spinal lumbar segments were not significantly changed between WT and TG mice at the pre-onset, onset, and progression timepoints (Figure 4).


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  • 发布日期: 2022-01-12  浏览: 339
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