脑损伤

    Ischemic accumulation of succinate induces Cdc42 succinylation and inhibits neural stem cell proliferation after cerebral ischemia/reperfusion
  • Figure 4|Succinate receptor G protein-coupled receptor 91 is unnecessary for diethyl succinate-mediated inhibition of C17.2 cell proliferation after oxygen-glucose deprivation/reoxygenation.  

    We confirmed the expression of the succinate receptor GPR91 in primary NSCs. The immunofluorescence assay showed that GPR91 and nestin colocalized in primary NSCs (Figure 4A). However, GPR91 expression was unaffected after OGD/R or DS treatment (Figure 4B and C). Western blot results showed that GPR91 expression was significantly increased in C17.2 cells after OGD/R compared with that in the negative control group (Figure 4D). Although DS inhibited cell proliferation, Gpr91 knockdown did not reverse cell proliferation inhibited by DS under physiological or OGD/R conditions (both P > 0.05; Figure 4E and F). Thus, GPR91 is not necessary for inhibition of C17.2 proliferation by DS.


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  • 发布日期: 2022-11-01  浏览: 137
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