中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (12): 2129-2133.doi: 10.4103/1673-5374.241464

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

黄芩苷对局灶性脑缺血的神经保护

  

  • 收稿日期:2018-06-26 出版日期:2018-12-15 发布日期:2018-12-15
  • 基金资助:

    本研究得到上海交通大学工程与医学跨学科重点项目（YG2016MS50）和仁济医院多学科临床研究培养基金（PYMDT-012）

Neuroprotective effect of baicalin on focal cerebral ischemia in rats

Jiong Dai1, Yong-Ming Qiu1, Zheng-Wen Ma2, Guo-Feng Yan2, Jing Zhou2, Shan-Quan Li1, Hui Wu1, Yi-Chao Jin1, Xiao-Hua Zhang1   

  1. 1 Department of Neurosurgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
    2 Department of of Laboratory Animal Science, Shanghai Jiao Tong University School of Medicine, Shanghai, China
  • Received:2018-06-26 Online:2018-12-15 Published:2018-12-15
  • Contact: Xiao-Hua Zhang, xiaohuazhang2@mcatom.cn.
  • Supported by:

    This study was supported by the Cross Foundation Major Project of Engineering and Medicine of Shanghai Jiao Tong University, No.YG2016MS50 (to JD) and Foundation for Fostering Project of Clinical Study on Multi-disciplinary Team of Renji Hospital, No. PYMDT-012 (to JD).

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摘要：

黄芩苷是来自黄芩根的黄酮类化合物，已广泛用于治疗炎性疾病。为进一步评估黄芩苷对局灶性脑缺血的干预效果，实验以大脑中动脉闭塞法建立局灶性脑缺血大鼠模型，然后将大鼠随机分为模型组（仅脑缺血再灌注）、丙戊酸组（脑缺血再灌注+腹腔注射丙戊酸3次；阳性对照）和黄芩苷组（脑缺血再灌注+腹腔注射黄芩苷21d）。在缺血后3,7,14和21d，使用姿势反射测试和前肢放置测试评估大鼠神经功能。缺血后第21天以TTC法检测大鼠脑梗死体积，以HE染色评估大鼠缺血脑组织病理改变。结果显示：（1）相较于缺血组，黄芩苷组大鼠神经功能明显改善，脑梗死体积明显缩小，缺血脑组织病理损害明显减轻；（2）且黄芩苷和丙戊酸组大鼠脑梗死体积相近；（3）实验结果数据表明黄芩苷对局灶性脑缺血有神经保护作用。

orcid:0000-0002-2192-4662(Xiao-Hua Zhang)

关键词: 脑缺血, 神经功能, 脑梗死体积, 大脑中动脉闭塞, 大鼠模型, 丙戊酸, 神经再生

Abstract:

Baicalin, a flavonoid compound from the root of the herb Scutellaria baicalensis Georgi, has been widely used to treat patients with inflammatory disease. The aim of this study was to assess the efficacy of baicalin in a rat model of focal cerebral ischemia. Adult male Sprague-Dawley rat models of cerebral artery occlusion were established and then randomly and equally divided into three groups: ischemia (cerebral ischemia and reperfusion), valproic acid (cerebral ischemia and reperfusion + three intraperitoneal injections of valproic acid; positive control), and baicalin (cerebral ischemia and reperfusion + intraperitoneal injection of baicalin for 21 days). Neurological deficits were assessed using the postural reflex test and forelimb placing test at 3, 7, 14, and 21 days after ischemia. Rat cerebral infarct volume was measured using 2,3,5-triphenyltetrazolium chloride (TTC) staining method. Pathological change of ischemic brain tissue was assessed using hematoxylin-eosin staining. In the baicalin group, rat neurological function was obviously improved, cerebral infarct volume was obviously reduced, and the pathological impairment of ischemic brain tissue was obviously alleviated compared to the ischemia group. Cerebral infarct volume was similar in the valproic acid and baicalin groups. These findings suggest that baicalin has a neuroprotective effect on cerebral ischemia.

Key words: cerebral ischemia, neurological function, cerebral infarct volume, middle cerebral artery occlusion, rat model, valproic acid, neural regeneration