中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (7): 1229-1234.doi: 10.4103/1673-5374.301018

• 原著:脑损伤修复保护与再生 •    下一篇

经颅脉冲电刺激可改善脑卒中大鼠的运动功能

  

  • 出版日期:2021-07-15 发布日期:2021-01-07
  • 基金资助:

    国家重点研发计划项目(2018YFC2001600);上海市卫健委加快中医药发展三年行动计划项目(ZY(2018-2020)-CCCX-2001-06/2004-05);上海市学术研究带头人计划(19XD1403600);国家自然科学基金青年项目(81704163)

Transcranial pulse current stimulation improves the locomotor function in a rat model of stroke

Wen-Jing Wang1, 2, #, Yan-Biao Zhong1, #, Jing-Jun Zhao2, 3, #, Meng Ren2, Si-Cong Zhang1, 2, Ming-Shu Xu4, Shu-Tian Xu2, Ying-Jie Zhang4, Chun-Lei Shan1, 2, *   

  1. 1 Center of Rehabilitation, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China;  2 School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China;  3 Department of Rehabilitation Medicine, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan Province, China;  4 Laboratory of Neurobiology, Shanghai Research Institute of Acupuncture and Meridian, Shanghai, China
  • Online:2021-07-15 Published:2021-01-07
  • Contact: Chun-Lei Shan, PhD, shanclhappy@163.com.
  • Supported by:

    This work was supported by the National Key R&D Program of China, No. 2018YFC2001600 (to CLS); the Shanghai Health Commission Accelerated the Development of Traditional Chinese Medicine Three-Year Action Plan Project, No. ZY(2018-2020)-CCCX-2001-06/2004-05 (to CLS); the Program of Shanghai Academic Research Leader, No. 19XD1403600 (to CLS); and the National Natural Science Foundation of China for the Youth Project, No. 81704163 (to JJZ).

摘要:

既往研究显示,经颅脉冲电刺激可提升大脑的神经可塑性,同时改善患者的运动功能,但其具体机制尚有待研究。实验以右侧大脑中动脉闭塞方法建立脑卒中大鼠模型,48h后行经颅脉冲电刺激,20min/次,1次/d,持续7d。结果见经颅脉冲电刺激可明显降低脑卒中大鼠的Bederson评分,增加患肢足迹面积,减少患肢站立时间;免疫荧光染色和Western blot结果显示其经颅脉冲电刺激能显著增加缺血半暗带周围微管相关蛋白2和生长相关蛋白43表达;表明经颅脉冲电刺激可通过影响缺血半暗带周围增微管相关蛋白2和生长相关蛋白43表达,改善脑卒中大鼠的运动功能。实验于2019年2月22日经上海中医药大学动物伦理委员会批准,批准号PZSHUTCM190315003。

https://orcid.org/0000-0002-8740-0538 (Wen-Jing Wang)

关键词: 脑卒中, 神经可塑性, 经颅电刺激, 非侵入性刺激, 运动功能, 修复, 保护

Abstract: Previous studies have shown that transcranial pulse current stimulation (tPCS) can increase cerebral neural plasticity and improve patients’ locomotor function. However, the precise mechanisms underlying this effect remain unclear. In the present study, rat models of stroke established by occlusion of the right cerebral middle artery were subjected to tPCS, 20 minutes per day for 7 successive days. tPCS significantly reduced the Bederson score, increased the foot print area of the affected limbs, and reduced the standing time of affected limbs of rats with stroke compared with that before intervention. Immunofluorescence staining and western blot assay revealed that tPCS significantly increased the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra. This finding suggests that tPCS can improve the locomotor function of rats with stroke by regulating the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra. These findings may provide a new method for the clinical treatment of poststroke motor dysfunction and a theoretical basis for clinical application of tPCS. The study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine of China (approval No. PZSHUTCM190315003) on February 22, 2019. 

Key words: motor function, neural plasticity, non-invasive brain stimulation, protection, repair, stroke, transcranial pulse current stimulation