中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (7): 1302-1307.doi: 10.4103/1673-5374.301019

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

pri-let-7a-2多态性与中国辽宁省人群缺血性卒中风险的相关病例对照

  

  • 出版日期:2021-07-15 发布日期:2021-01-07
  • 基金资助:

    国家自然科学基金项目(81901189),辽宁省自然科学基金项目(2019-BS-147

Genetic polymorphisms in pri-let-7a-2 are associated with ischemic stroke risk in a Chinese Han population from Liaoning, China: a case-control study

Yu-Ye Wang1, #, He-Yu Zhang2, #, Wen-Juan Jiang1, 3, Fang Liu1, Lei Li1, Shu-Min Deng1, Zhi-Yi He1, Yan-Zhe Wang1, 3, *   

  1. 1 Department of Neurology, Key Laboratory for Neurological Big Data of Liaoning Province, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China;  2 Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University; Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, Guangzhou, Guangdong Province, China;  3 Department of Pharmacology, School of Pharmacy, China Medical University, Shenyang, Liaoning Province, China
  • Online:2021-07-15 Published:2021-01-07
  • Contact: Yan-Zhe Wang, MD, PhD, yanzhewangcmu@126.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81901189, and the Natural Science Foundation of Liaoning Province of China, No. 2019-BS-147 (both to YZW).

摘要:

缺血性卒中是一种复杂的疾病,遗传多态性可能对其发病起关键作用。有证据表明,microRNA let-7a可参与了缺血性卒中的发病机制。此次病例对照研究拟分析易感性缺血性卒中患者pri-let-7a-2 基因多态性的分布以及其与环境因素的相互作用。于201312月至201512月在中国医科大学附属第一医院收集了1086例缺血性卒中患者和836名健康对照者的血液样本和临床信息。发现rs1143770 CC基因型和C等位基因与缺血性卒中风险降低有关,而rs629367 CC基因型与缺血性卒中风险增加有关。且2种单核苷酸多态性处于连锁不平衡中。通过对基因-环境相互作用的分析发现,rs1143770与酗酒,吸烟和高血压病史可能在缺血性卒中的发病机理中起着联合作用。因此认为pri-let-7a-2多态性会影响缺血性卒中的风险。试验于2012220日经中国医科大学附属第一医院伦理委员会批准,批准号2012-38-1,并于20171227日在中国临床试验注册中心注册,注册号:ChiCTR- COC-17013559

https://orcid.org/0000-0003-1330-6298 (Yan-Zhe Wang)

关键词:

缺血性脑卒中, pri-microRNA, pri-microRNA-let-7a-2, 单核苷酸多态性, 单核苷酸多态性-环境相互作用, rs1143770, rs629367, 病例对照研究, 中国汉族, 风险

Abstract: Ischemic stroke is a complicated disease, and its pathogenesis has been attributed to the occurrence of genetic polymorphisms. Evidence has suggested that the microRNA let-7a is involved in the pathogenesis of ischemic stroke. Pri-miRNA is the primary transcript, which undergoes several processing steps to generate pre-miRNA and, later, mature miRNAs. In this case-control study, we analyzed the distribution of pri-let-7a-2 variants in patients at a high risk for ischemic stroke and the interactions of pri-let-7a-2 variants and environmental factors. Blood samples and clinical information were collected from 1086 patients with ischemic stroke and 836 healthy controls between December 2013 and December 2015 at the First Affiliated Hospital of China Medical University. We found that the rs1143770 CC genotype and the C allele were associated with a decreased risk of ischemic stroke, whereas the rs629367 CC genotype was associated with an increased risk for ischemic stroke. Moreover, these two single-nucleotide polymorphisms were in linkage disequilibrium in this study sample. We analyzed gene-environment interactions and found that rs1143770 exerted a combined effect on the pathogenesis of ischemic stroke, together with alcohol use, smoking, and a history of hypertension. Therefore, the detection of pri-let-7a-2 polymorphisms may increase the awareness of ischemic stroke risk. This study was approved by the Institutional Ethics Committee of the First Affiliated Hospital of China Medical University, China (approval No. 2012-38-1) on February 20, 2012, and was registered with the Chinese Clinical Trial Registry (registration number: ChiCTR-COC-17013559) on December 27, 2017.

Key words: case-control study, Chinese Han population, ischemic stroke, pri-microRNA, pri- let-7a-2, risk factors, rs1143770, rs629367, single-nucleotide polymorphism, single-nucleotide polymorphism-environment interaction