中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1622-1627.doi: 10.4103/1673-5374.301025

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

坐骨神经损伤模型大鼠早期神经修复相关蛋白的自然变化

  

  • 出版日期:2021-08-15 发布日期:2021-01-13
  • 基金资助:

    国家重点研究发展计划项目(2016YFC1101604);中央高校基本科研业务费专项资金资助-临床医学+X-北京大学青年学者计划(PKU2020LCXQ020);教育部创伤与神经再生重点实验室(北京大学)(BMU2019XY007-01);广东省基础和应用基础研究基金(2019A15151109832019A1515011290);深圳“三明”医学项目(SZSM201612092

Changes in proteins related to early nerve repair in a rat model of sciatic nerve injury

Yu-Song Yuan1, 2, #, Fei Yu1, 3, #, Ya-Jun Zhang4, Su-Ping Niu5, Hai-Lin Xu1, 6, *, Yu-Hui Kou1, 2, *   

  1. 1Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China; 2Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, Beijing, China; 3National & Local Joint Engineering Research Center of Orthopedic Biomaterials, Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China; 4National Center for Trauma Medicine, Beijing, China; 5Office of Academic Research, Peking University People’s Hospital, Beijing, China; 6Diabetic Foot Treatment Center, Peking University People’s Hospital, Beijing, China
  • Online:2021-08-15 Published:2021-01-13
  • Contact: Hai-Lin Xu, MD, xuhailinfa@163.com; Yu-Hui Kou, PhD, yuhuikou@bjmu.edu.cn.
  • Supported by:
    This study was supported by the National Key Research and Development Program of China, No. 2016YFC1101604 (to YHK); the Fundamental Research Funds for the Central Universities, Clinical Medicine Plus X - Young Scholars Project of Peking University, No. PKU2020LCXQ020 (to YHK); the Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education of China, No. BMU2019XY007-01 (to YHK); Guangdong Basic and Applied Basic Research Foundation of China, Nos. 2019A1515110983 (to FY) and 2019A1515011290 (to FY); and Shenzhen “San-Ming” Project of Medicine of China, No. SZSM201612092 (to FY).

摘要:

周围神经的自我修复能力有限,严重损伤或缺损的周围神经很难修复,因而研究周围神经修复的病理生理机制,对于周围神经修复和再生的临床治疗至关重要。此次实验以夹闭法建立了右侧坐骨神经损伤大鼠模型,以蛋白芯片定量分析损伤7d内坐骨神经中神经营养因子、炎症相关因子、趋化性相关因子和细胞生成相关因子的表达变化。结果发现损伤后,大鼠坐骨神经组织中神经营养因子(睫状神经营养因子)、炎症相关因子(细胞间细胞粘附分子1、干扰素γ、白细胞介素1α、白细胞介素2、白细胞介素4、白细胞介素6、单核细胞趋化蛋白1、催乳素R、晚期糖化终产物受体、肿瘤坏死因子α)、趋化性相关因子(中性粒细胞趋化因子1L-选择素、血小板衍生的生长因子AA)和细胞生成相关因子(粒细胞-巨噬细胞集落刺激因子)出现不同水平的变化。这将为阐明坐骨神经修复这一病理生理过程的机制以及临床周围神经损伤的治疗策略的制定提供帮助。实验于2015129日经北京大学人民医院伦理委员会批准,批准号为2015-50

https://orcid.org/0000-0003-1829-3852 (Hai-Lin Xu); 

https://orcid.org/0000-0003-1523-825X (Yu-Hui Kou)

关键词: 坐骨神经, 神经元夹伤, 动物模型, 蛋白阵列, 修复, 损伤, Wallerian变性, 周围神经

Abstract: Peripheral nerves have a limited capacity for self-repair and those that are severely damaged or have significant defects are challenging to repair. Investigating the pathophysiology of peripheral nerve repair is important for the clinical treatment of peripheral nerve repair and regeneration. In this study, rat models of right sciatic nerve injury were established by a clamping method. Protein chip assay was performed to quantify the levels of neurotrophic, inflammation-related, chemotaxis-related and cell generation-related factors in the sciatic nerve within 7 days after injury. The results revealed that the expression levels of neurotrophic factors (ciliary neurotrophic factor) and inflammation-related factors (intercellular cell adhesion molecule-1, interferon γ, interleukin-1α, interleukin-2, interleukin-4, interleukin-6, monocyte chemoattractant protein-1, prolactin R, receptor of advanced glycation end products and tumor necrosis factor-α), chemotaxis-related factors (cytokine-induced neutrophil chemoattractant-1, L-selectin and platelet-derived growth factor-AA) and cell generation-related factors (granulocyte-macrophage colony-stimulating factor) followed different trajectories. These findings will help clarify the pathophysiology of sciatic nerve injury repair and develop clinical treatments of peripheral nerve injury. This study was approved by the Ethics Committee of Peking University People’s Hospital of China (approval No. 2015-50) on December 9, 2015. 

Key words: animal model, cell generation, chemotaxis, clamp injury, inflammation, injury, neurotrophic factor, peripheral nerve protein array, repair, sciatic nerve, Wallerian degeneration