中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (7): 1323-1330.doi: 10.4103/1673-5374.301486

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

体感和运动诱发电位可反映单侧颈部脊髓挫伤后粗大和精细运动的功能

  


  • 出版日期:2021-07-15 发布日期:2021-01-07
  • 基金资助:

    国家自然科学基金面上项目（81871768）；中国医学科学院创新医学计划（2016-I2M-2-006）；广东省卫健委高等级医院计划项目（HKUSZH201902011）

Utility of somatosensory and motor-evoked potentials in reflecting gross and fine motor functions after unilateral cervical spinal cord contusion injury

Rong Li1, 4, Zu-Cheng Huang2, Hong-Yan Cui1, Zhi-Ping Huang2, Jun-Hao Liu2, Qing-An Zhu2, *, Yong Hu1, 3, 4, *   

  1. 1 Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China;  2 Department of Spine Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China;  3 Department of Orthopedics and Traumatology, The University of Hong Kong, Hong Kong Special Administrative Region, China;  4 Department of Orthopedics and Traumatology, The Hong Kong University Shenzhen Hospital, Shenzhen, Guangdong Province, China

  • Online:2021-07-15 Published:2021-01-07
  • Contact: Yong Hu, PhD, yhud@hku.hk; Qing-An Zhu, PhD, qinganzhu@gmail.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81871768 (to YH); Chinese Academy of Medical Sciences Initiative for Innovative Medicine of China, No. 2016-I2M-2-006 (to HYC); and High Level-Hospital Program, Health Commission of Guangdong Province, China, No. HKUSZH201902011 (to YH).

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摘要：

精细运动依赖于脊髓感觉（上行）传导通路和起源于新皮质运动（下行）传导通路的完整性。然而，在感觉和运动通路之间的交流的生理过程，尤其是其参与脊髓损伤后自发恢复过程中调节精细运动的机制，仍然不明确。因此实验通过C5脊髓挫伤建立脊髓挫伤大鼠模型，并按挫伤至脊髓位移1.2mm或2.0mm分为轻度或重度脊髓挫伤。损伤后连续12周采集肱肌电生理数据，希望能够了解脊髓通路协调参与运动功能的机制。结果发现脊髓损伤后，大鼠的体感和运动诱发电位振幅减小，潜伏期增加，同时旷场测试、捋毛实验、直立测试以及Montoya楼梯实验结果明显改善。随损伤后时间的延长，轻度脊髓损伤大鼠体感和运动诱发电位振幅逐渐增加，同时潜伏期逐渐缩短，但重度脊髓损伤大鼠的体感和运动诱发电位振幅和潜伏期的恢复时间比轻度脊髓损伤大鼠要长，且运动功能的恢复比轻度脊髓损伤大鼠延迟。相关性分析结果显示，轻度损伤大鼠体感和运动诱发电位振幅和潜伏期与的粗大和精细运动功能有关；重度脊髓损伤大鼠只有体感诱发电位振幅与精细运动技能有关。提示体感和运动诱发电位可反映轻度脊髓挫伤后粗大和精细运动功能的变化，且体感诱发电位的变化还可反映重度脊髓挫伤后精细运动功能的变化。实验于2017-6-11经南方医科大学南方医院动物伦理委员会批准，批准号NFYY-2017- 67。

https://orcid.org/0000-0003-0305-5616 (Yong Hu); 

https://orcid.org/0000-0002-0412-0515 (Qing-An Zhu)

关键词: 中枢神经系统, 脊髓, 运动功能, 脊髓损伤, 体感诱发电位, 运动诱发电位, 修复, 再生

Abstract: Fine motor skills are thought to rely on the integrity of ascending sensory pathways in the spinal dorsal column as well as descending motor pathways that have a neocortical origin. However, the neurophysiological processes underlying communication between the somatosensory and motor pathways that regulate fine motor skills during spontaneous recovery after spinal cord contusion injury remain unclear. Here, we established a rat model of cervical hemicontusive injury using C5 laminectomy followed by contusional displacement of 1.2 mm (mild injury) or 2.0 mm (severe injury) to the C5 spinal cord. Electrophysiological recordings were performed on the brachial muscles up to 12 weeks after injury to investigate the mechanisms by which spinal cord pathways participate in motor function. After spinal cord contusion injury, the amplitudes of somatosensory and motor-evoked potentials were reduced, and the latencies were increased. The forelimb open field locomotion test, grooming test, rearing test and Montoya staircase test revealed improvement in functions. With increasing time after injury, the amplitudes of somatosensory and motor-evoked potentials in rats with mild spinal cord injury increased gradually, and the latencies gradually shortened. In comparison, the recovery times of somatosensory and motor-evoked potential amplitudes and latencies were longer, and the recovery of motor function was delayed in rats with severe spinal cord injury. Correlation analysis revealed that somatosensory-evoked potential and motor-evoked potential parameters were correlated with gross and fine motor function in rats with mild spinal cord contusion injury. In contrast, only somatosensory-evoked potential amplitude was correlated with fine motor skills in rats with severe spinal cord injury. Our results show that changes in both somatosensory and motor-evoked potentials can reflect the changes in gross and fine motor functions after mild spinal cord contusion injury, and that the change in somatosensory-evoked potential amplitude can also reflect the change in fine motor function after severe spinal cord contusion injury. This study was approved by the Animal Ethics Committee of Nanfang Hospital, Southern Medical University, China (approval No. NFYY-2017-67) on June 11, 2017.

Key words: central nervous system, motor-evoked potential, motor function, regeneration, repair, somatosensory-evoked potential, spinal cord, spinal cord injury