中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1453-1459.doi: 10.4103/1673-5374.303033

• 原著:神经损伤修复保护与再生 •    下一篇

脑源性神经营养因子及相关酶和受体在脑缺氧缺血损伤后起重要作用

  

  • 出版日期:2021-08-15 发布日期:2021-01-13
  • 基金资助:

    国家自然科学基金面上项目(82001604);西南医科大学与西南医科大学附属中医院联合学科项目(2018XYLH-004);国家区域中医药治疗中心国家建设项目(2018205);国家中医药第二批临床研究基地国家建设项目(2018131

Brain-derived neurotrophic factor and its related enzymes and receptors play important roles after hypoxic-ischemic brain damage

Liu-Lin Xiong1, 2, #, Jie Chen1, #, Ruo-Lan Du3, Jia Liu3, Yan-Jun Chen4, Mohammed Al Hawwas2, Xin-Fu Zhou2, Ting-Hua Wang3, 4, Si-Jin Yang1, *, Xue Bai1, *   

  1. 1National Traditional Chinese Medicine Clinical Research Base and Western Medicine Translational Medicine Research Center, Department of Cardiovascular Disease, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan Province, China; 2Cinical and Health Sciences, University of South Australia, Adelaide, Australia; 3Animal Zoology Department, Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China; 4Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
  • Online:2021-08-15 Published:2021-01-13
  • Contact: Xue Bai, MS, XueBai159263487@163.com; Si-Jin Yang, MS, ysjimn@sina.com.
  • Supported by:
     This study was supported by the National Natural Science Foundation of China, No. 82001604 (to LLX); the Joint Subject of Southwest Medical University and Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University of China, No. 2018XYLH-004 (to LLX); the National Construction Project of Regional Chinese Medicine Treatment Centre of China, No. 2018205 (to XB); the National Construction Project of the Second Clinical Research Base of Chinese Medicine of China, No. 2018131 (to XB). 

摘要:

脑源性神经营养因子可调节多种神经功能,且在损伤中枢神经系统的恢复中起至关重要的作用。然而新生儿脑缺氧缺血损伤中脑源性神经营养因子表达变化情况以其意义尚不完全清楚。实验电凝新生大鼠右侧颈总动脉后低氧饲养2h建立缺氧缺血脑损伤模型,发现脑缺氧缺血大鼠出现感觉和运动功能缺失,且脑组织发生了明显的病理改变,同时在损伤侧海马和大脑皮质中,脑源性神经营养因子及其加工酶和受体(Furin,基质金属蛋白酶9,组织型纤溶酶原激活剂,TrkB,PAI-1,Sortilin)mRNA表达在损伤后6h时均上调,但却在对侧大脑皮质中表达下降。因此提示脑源性神经营养因子及其加工酶和受体可能在新生儿缺氧缺血脑损伤的发病和恢复中起重要作用。实验于2018年7月30日经UNISA动物伦理委员会批准,批准号U12-18。

https://orcid.org/0000-0002-2040-7778 (Xue Bai)

关键词: 脑源性神经营养因子, 缺氧缺血, 酶, 受体, 脑损伤, 修复, 恢复

Abstract: Brain-derived neurotrophic factor (BDNF) regulates many neurological functions and plays a vital role during the recovery from central nervous system injuries. However, the changes in BDNF expression and associated factors following hypoxia-ischemia induced neonatal brain damage, and the significance of these changes are not fully understood. In the present study, a rat model of hypoxic-ischemic brain damage was established through the occlusion of the right common carotid artery, followed by 2 hours in a hypoxic-ischemic environment. Rats with hypoxic-ischemic brain damage presented deficits in both sensory and motor functions, and obvious pathological changes could be detected in brain tissues. The mRNA expression levels of BDNF and its processing enzymes and receptors (Furin, matrix metallopeptidase 9, tissue-type plasminogen activator, tyrosine Kinase receptor B, plasminogen activator inhibitor-1, and Sortilin) were upregulated in the ipsilateral hippocampus and cerebral cortex 6 hours after injury; however, the expression levels of these mRNAs were found to be downregulated in the contralateral hippocampus and cerebral cortex. These findings suggest that BDNF and its processing enzymes and receptors may play important roles in the pathogenesis and recovery from neonatal hypoxic-ischemic brain damage. This study was approved by the Animal Ethics Committee of the University of South Australia (approval No. U12-18) on July 30, 2018. 

Key words: brain injury, brain-derived neurotrophic factor, enzyme, hypoxia-ischemia, receptors, recovery, repair