中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (3): 601-607.doi: 10.4103/1673-5374.320987

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

确定脊髓损伤大鼠硬膜外脊髓刺激运动功能区域柔性电极阵列设计

  

  • 出版日期:2022-03-15 发布日期:2021-10-15

A flexible electrode array for determining regions of motor function activated by epidural spinal cord stimulation in rats with spinal cord injury

Guang-Wei Mao1, Jian-Jun Zhang1, Hao Su1, Zhi-Jun Zhou2, Lin-Sen Zhu2, Xiao-Ying Lü1, 3, *, Zhi-Gong Wang2, 3, *#br#   

  1. 1State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu Province, China; 2Institute of RF- & OE-ICs, Southeast University, Nanjing, Jiangsu Province, China; 3Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
  • Online:2022-03-15 Published:2021-10-15
  • Contact: Xiao-Ying Lü, PhD, luxy@seu.edu.cn; Zhi-Gong Wang, PhD, zgwang@seu.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, Nos. 61534003 (to ZGW) and 61874024 (to ZGW).

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摘要：

硬膜外脊髓电刺激是脊髓损伤后恢复运动功能的一项很有前景的治疗方法，瘫痪肢体重建运动功的关键在于精确控制硬膜外脊髓电刺激的刺激部位和参数。为了通过硬膜外刺激精确激活下肢肌肉，该课题组设计了一种高密度柔性电极阵列，并希望在脊髓损伤模型大鼠上定位硬膜外刺激运动功能区。(1)实验以横断法制备脊髓损伤模型大鼠，为评估硬膜外刺激的效果，记录了脊髓损伤模型大鼠双侧下肢肌肉的诱发电位，包括股外侧肌、半腱肌、胫前肌和腓肠肌内侧肌。为了确定合适的刺激部位和参数，在硬膜外刺激运动功能区内研究了刺激的特性；(2)脊髓损伤模型大鼠的股外侧肌和腓肠肌内侧肌的硬膜外刺激运动功能区分别位于L1椎体外侧和L2椎体内侧，胫前肌和半腱肌的刺激响应只与其他肌肉同时出现，股外侧肌的刺激阈值电流高于腓肠肌内侧肌；(3)上述数据证实，此高密度柔性阵列能够识别出大鼠下肢肌肉的特定刺激位置，为不同脊髓损伤动物模型植入性刺激参数和位置的选择提供了参考。实验于2019-07-20获得中国东南大学动物研究委员会批准(批准号：20190720001)。

https://orcid.org/0000-0002-3777-2472 (Xiao-Ying Lü); https://orcid.org/0000-0002-9203-4683 (Zhi-Gong Wang) 

关键词: 硬膜外脊髓刺激, 诱发电位, 高密度柔性电极阵列, 运动功能, 大鼠, 康复, 脊髓损伤, 脊髓节段, 刺激参数, 刺激反应

Abstract: Epidural stimulation of the spinal cord is a promising technique for the recovery of motor function after spinal cord injury. The key challenges within the reconstruction of motor function for paralyzed limbs are the precise control of sites and parameters of stimulation. To activate lower-limb muscles precisely by epidural spinal cord stimulation, we proposed a high-density, flexible electrode array. We determined the regions of motor function that were activated upon epidural stimulation of the spinal cord in a rat model with complete spinal cord, which was established by a transection method. For evaluating the effect of stimulation, the evoked potentials were recorded from bilateral lower-limb muscles, including the vastus lateralis, semitendinosus, tibialis anterior, and medial gastrocnemius. To determine the appropriate stimulation sites and parameters of the lower muscles, the stimulation characteristics were studied within the regions in which motor function was activated upon spinal cord stimulation. In the vastus lateralis and medial gastrocnemius, these regions were symmetrically located at the lateral site of L1 and the medial site of L2 vertebrae segment, respectively. The tibialis anterior and semitendinosus only responded to stimulation simultaneously with other muscles. The minimum and maximum stimulation threshold currents of the vastus lateralis were higher than those of the medial gastrocnemius. Our results demonstrate the ability to identify specific stimulation sites of lower muscles using a high-density and flexible array. They also provide a reference for selecting the appropriate conditions for implantable stimulation for animal models of spinal cord injury. This study was approved by the Animal Research Committee of Southeast University, China (approval No. 20190720001) on July 20, 2019. 

Key words: electrode array, epidural spinal cord stimulation, evoked potentials, motor function, rehabilitation, spinal cord injury, spinal segment

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