中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (4): 838-844.doi: 10.4103/1673-5374.322468

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

海马弥散张量成像可评估全脑缺血再灌注损伤致海马损伤的严重程度

  

  • 出版日期:2022-04-15 发布日期:2021-10-18

Diffusion tensor imaging of the hippocampus reflects the severity of hippocampal injury induced by global cerebral ischemia/reperfusion injury

Wen-Zhu Wang1, 2, Xu Liu3, Zheng-Yi Yang4, Yi-Zheng Wang1, Hai-Tao Lu1, *   

  1. 1China Rehabilitation Science Institute, School of Rehabilitation Medicine, Capital Medical University, Beijing Bo’ai Hospital, China Rehabilitation Research Center, Beijing, China; 2Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing, China; 3Department of Rehabilitation Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing, China; 4Brainnetome Center, Institute of Automation, Chinese Academy of Sciences, Beijing, China
  • Online:2022-04-15 Published:2021-10-18
  • Contact: Hai-Tao Lu, PhD, 13051760807@163.com.
  • Supported by:
    This study was supported by the Fundamental Research Funds for Central Public Welfare Research Institute of China, Nos. 2015CZ-36 (to HTL) and 2019CZ-7 (to WZW).

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摘要：

目前对全脑缺血再灌注损伤所致脑损伤严重程度的预判是临床难题之一，且临床上也缺乏可直接反映全脑缺血再灌注损伤后神经功能障碍的检测指标。海马亚区微结构变化是记忆形成与巩固的关键，而弥散张量成像对海马微结构的损伤十分敏感。虽然全脑缺血再灌注损伤后，海马亚区微结构、海马脑源性神经营养因子与TrkB水平与神经损伤与修复过程紧密相关，但是尚不清楚在上述指标是否能反映由全脑缺血再灌注损伤引起的海马损伤的严重程度。为解答这一问题，实验以四血管阻塞法建立全脑缺血再灌注损伤大鼠模型，分析弥散张量成像参数、脑源性神经营养因子和TrkB水平与改良神经系统严重程度评分的相关性。结果发现全脑缺血再灌注损伤后血浆脑源性神经营养因子和TrkB水平与神经功能无关，而海马各向异性分数与神经功能有关。从而表明海马各向异性分数可反映全脑缺血再灌注损伤后海马损伤程度。实验于2015年11月9日经首都医科大学动物伦理委员会批准（批准号AEEI-2015-139）。

https://orcid.org/0000-0002-1775-9780 (Hai-Tao Lu)

关键词: 弥散张量成像, 脑源性神经营养因子, TrkB, 全脑缺血再灌注损伤, 海马, 血浆, 各向异性分数, 灰质密度

Abstract: At present, predicting the severity of brain injury caused by global cerebral ischemia/reperfusion injury (GCI/RI) is a clinical problem. After such an injury, clinical indicators that can directly reflect neurological dysfunction are lacking. The change in hippocampal microstructure is the key to memory formation and consolidation. Diffusion tensor imaging is a highly sensitive tool for visualizing injury to hippocampal microstructure. Although hippocampal microstructure, brain-derived neurotrophic factor (BDNF), and tropomyosin-related kinase B (TrkB) levels are closely related to nerve injury and the repair process after GCI/RI, whether these indicators can reflect the severity of such hippocampal injury remains unknown. To address this issue, we established rat models of GCI/RI using the four-vessel occlusion method. Diffusion tensor imaging parameters, BDNF, and TrkB levels were correlated with modified neurological severity scores. The results revealed that after GCI/RI, while neurological function was not related to BDNF and TrkB levels, it was related to hippocampal fractional anisotropy. These findings suggest that hippocampal fractional anisotropy can reflect the severity of hippocampal injury after global GCI/RI. The study was approved by the Institutional Animal Care and Use Committee of Capital Medical University, China (approval No. AEEI-2015-139) on November 9, 2015.

Key words: brain-derived neurotrophic factor, diffusion tensor imaging, fractional anisotropy value, global cerebral ischemia/reperfusion injury, hippocampus, TrkB 

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