中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (7): 1512-1517.doi: 10.4103/1673-5374.330616

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

全脑缺血期间低温可选择性保护前脑中间回路

  

  • 出版日期:2022-07-15 发布日期:2022-01-17
  • 基金资助:

    北京市卫健委项目(Jing2019-2

Hypothermia selectively protects the anterior forebrain mesocircuit during global cerebral ischemia

Xiao-Hua Wang1, 2, #, Wei Jiang3, #, Si-Yuan Zhang4, Bin-Bin Nie5, 6, Yi Zheng7, Feng Yan8, Jian-Feng Lei8, Tian-Long Wang1, 2, *   

  1. 1Department of Anesthesiology, Xuanwu Hospital, Capital Medical University, Beijing, China; 2National Clinical Research Center for Geriatric Disorders, Beijing, China; 3Department of Anesthesiology, Third Medical Center of People’s Liberation Army General Hospital, Beijing, China; 4Daxing Hospital Affiliated to Capital Medical University, Beijing, China; 5Beijing Engineering Research Center of Radiographic Techniques and Equipment, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China; 6School of Nuclear Science and Technology, University of Chinese Academy of Sciences, Beijing, China; 7Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, USA; 8Cerebrovascular Research Center, Xuanwu Hospital, Capital Medical University, Beijing, China
  • Online:2022-07-15 Published:2022-01-17
  • Contact: Tian-Long Wang, PhD, w_tl5595@yahoo.com.
  • Supported by:
    This study was supported by Beijing Municipal Health Commission of China, No. Jing2019-2 (to TLW).

摘要:

低温是防止心脏骤停后全脑缺血的重要保护策略,然而低温导致不同脑区和大脑连接变化的机制尚在研究中。此次研究拟使用18F-氟代脱氧葡萄糖正电子发射断层扫描定量测定31–33°C低温环境下建立的全脑缺血大鼠模型不同脑区中的葡萄糖代谢。此外还使用了弥散张量纤维束成像重建和探索受低温保护的大脑连接,以研究低温对全脑缺血模型大鼠特定脑区回路代谢节点和连接完整性的作用。结果显示,与常温(37–37.5°C)建立的模型大鼠相比,低温环境建立的全脑缺血模型大鼠前脑中枢回路中丘脑及初级感觉区中低代谢区域明显较小,其丘脑损伤不明显;体素分析显示,低温选择性地保留了包含丘脑、前额叶皮质以及胼胝体和内囊间连接在内的前脑前部中间回路。显示低温通过选择性保护前脑中间回路的完整性,从而在全脑缺血期间发挥脑保护作用。实验于2019年12月15日经首都医科大学动物伦理委员会批准(批准号XW-AD318-97-019)。

https://orcid.org/0000-0003-1636-0142 (Tian-Long Wang)

关键词:

低温, 前脑中间回路, 全脑缺血, 心脏骤停, 正电子发射断层扫描, 磁共振成像, 丘脑, 脑保护

Abstract: Hypothermia is an important protective strategy against global cerebral ischemia following cardiac arrest. However, the mechanisms of hypothermia underlying the changes in different regions and connections of the brain have not been fully elucidated. This study aims to identify the metabolic nodes and connection integrity of specific brain regions in rats with global cerebral ischemia that are most affected by hypothermia treatment. 18F-fluorodeoxyglucose positron emission tomography was used to quantitatively determine glucose metabolism in different brain regions in a rat model of global cerebral ischemia established at 31–33°C. Diffusion tensor imaging was also used to reconstruct and explore the brain connections involved. The results showed that, compared with the model rats established at 37–37.5°C, the rat models of global cerebral ischemia established at 31–33°C had smaller hypometabolic regions in the thalamus and primary sensory areas and sustained no obvious thalamic injury. Hypothermia selectively preserved the integrity of the anterior forebrain mesocircuit, exhibiting protective effects on the brain during the global cerebral ischemia. The study was approved by the Institutional Animal Care and Use Committee at Capital Medical University (approval No. XW-AD318-97-019) on December 15, 2019.

Key words: anterior forebrain mesocircuit, cardiac arrest, corpus callosum, global cerebral ischemia, hypometabolic areas, hypothermia, magnetic resonance imaging, positron emission tomography, prefrontal cortex, rats, thalamus