中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (8): 1761-1768.doi: 10.4103/1673-5374.331539

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

泛死亡是否存在于脑缺血性损伤中?源自细胞和啮齿动物实验的证据挖掘

  

  • 出版日期:2022-08-15 发布日期:2022-01-22
  • 基金资助:
    国家自然科学基金项目(81772134,81971891,82172196,81571939);中南大学中央高校基本科研业务费专项资金(2020zzts218);湖南省研究生创新基金(CX20200116,CX20190139)

Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies

Wei-Tao Yan1, Yan-Di Yang1, Xi-Min Hu2, Wen-Ya Ning3, Lyu-Shuang Liao1, Shuang Lu1, Wen-Juan Zhao1, Qi Zhang1, *, Kun Xiong1, 4, *   

  1. 1Department of Neurobiology and Human Anatomy, School of Basic Medical Science, Central South University, Changsha, Hunan Province, China; 2Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan Province, China; 3Department of Human Resources, Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China; 4Hunan Key Laboratory of Ophthalmology, Changsha, Hunan Province, China
  • Online:2022-08-15 Published:2022-01-22
  • Contact: Kun Xiong, MD, xiongkun2001@163.com; Qi Zhang, MD, zhangqi2014@csu.edu.cn.
  • Supported by:
    The study was supported by the National Natural Science Foundation of China, Nos. 81772134 (to KX), 81971891 (to KX), 82172196 (to KX), 81571939 (to KX); the Fundamental Research Funds for the Central Universities of Central South University of China, No. 2020zzts218, (to WTY); Hunan Provincial Innovation Foundation For Postgraduate of China, Nos. CX20200116 (to WTY), CX20190139 (to LSL).

摘要:

在感染性疾病研究中提出了泛死亡的概念,认为细胞焦亡、细胞凋亡和程序性坏死可共同构成泛死亡,三者可同时受到多分子复合体PANoptosome的调控。这些有关泛死亡的新发现为可调节性细胞死亡的研究提供了新思路,即不同类型的可调节性细胞死亡可能同时被干预。同时也引发了一些思考,即泛死亡是否也存在于其他疾病中?为研究这一问题,研究选择脑缺血再灌注损伤作为研究对象,从数据库中收集有关脑缺血再灌注的研究,通过文献计量学、知识发现和数据挖掘方法获取原始数据,并对其进行整合分析,探索脑缺血再灌注损伤中存在泛死亡的可能。在模拟缺血性脑损伤的细胞模型中,同时存在细胞焦亡、凋亡和程序性坏死,且这种现象在不同的传代细胞系或原代神经元中都广泛存在。同时,在大鼠和小鼠脑缺血再灌注损伤模型中,也存在细胞焦亡、凋亡和程序性坏死发生的证据。这证明了泛死亡可能存在于缺血性脑损伤,并预示着在中枢神经系统疾病中,细胞的泛死亡有可能参与了各种疾病的调控。

https://orcid.org/0000-0002-3103-6028 (Kun Xiong); https://orcid.org/0000-0001-6300-6491 (Qi Zhang);

https://orcid.org/0000-0002-2561-9673 (Wei-Tao Yan)

关键词: 泛死亡, 细胞焦亡, 细胞凋亡, 程序性坏死, 缺血再灌注, 中枢神经系统, 脑, 可调控性细胞死亡, 大脑中动脉闭塞, 氧糖剥夺

Abstract: Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis, apoptosis and necroptosis act in consort in a multimeric protein complex, PANoptosome. This allows all the components of PANoptosis to be regulated simultaneously. PANoptosis provides a new way to study the regulation of cell death, in that different types of cell death may be regulated at the same time. To test whether PANoptosis exists in diseases other than infectious diseases, we chose cerebral ischemia/reperfusion injury as the research model, collected articles researching cerebral ischemia/reperfusion from three major databases, obtained the original research data from these articles by bibliometrics, data mining and other methods, then integrated and analyzed these data. We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion. In the cell model simulating ischemic brain injury, pyroptosis, apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons. Pyroptosis, apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury. This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases.

Key words: apoptosis, brain, central nervous system, ischemia/reperfusion, middle cerebral artery occlusion, necroptosis, oxygen and glucose deprivation, PANoptosis, pyroptosis, regulated cell death