中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (1): 162-169.doi: 10.4103/1673-5374.344842

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

神经生长因子-碱性成纤维生长因子-聚丙交酯-共-乙交酯缓释微球联合小间隙套管桥接技术修复周围神经损伤

  

  • 出版日期:2023-01-15 发布日期:2022-06-17
  • 基金资助:
    国家重点研发计划项目（2016YFC1101603）；国家自然科学基金项目（31640045，81901251）；北京市自然科学基金项目（7204323）

Nerve growth factor-basic fibroblast growth factor poly-lactide co-glycolid sustained-release microspheres and the small gap sleeve bridging technique to repair peripheral nerve injury

Ming Li1, 2, 3, #, Ting-Min Xu1, 2, #, Dian-Ying Zhang1, #, Xiao-Meng Zhang1, Feng Rao2, 3, Si-Zheng Zhan1, Man Ma1, Chen Xiong1, Xiao-Feng Chen1, Yan-Hua Wang1, *, #   

  1. 1Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China; 2Key Laboratory of Trauma and Neural Regeneration (Peking University), Ministry of Education, Beijing, China; 3Trauma Medicine Center, Peking University People’s Hospital; National Center for Trauma Medicine, Beijing, China
  • Online:2023-01-15 Published:2022-06-17
  • Contact: Yan-Hua Wang, PhD, wangyanhua04119@pkuph.edu.cn.
  • Supported by:
    This work was supported by the National Key Research and Development Program of China, No. 2016YFC1101603 (to DYZ); the National Natural Science Foundation of China, Nos. 31640045 (to YHW), 81901251 (to ML); the Natural Science Foundation of Beijing of China, No. 7204323 (to ML).

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摘要：

作者既往研究已制备了一种神经生长因子-聚丙交酯-共-乙交酯缓释微球，可通过结合小间隙套筒桥接修复大鼠坐骨神经损伤。由于多种生长因子联合可在促进周围神经损伤修复方面发挥协同作用，因而实验拟探索将上述微球中加入碱性成纤维生长因子，以进一步加强其促神经再生的效果。为此，首先在体外仿生微环境中，利用药物筛选仿生微流控芯片筛选出神经生长因子和碱性成纤维细胞生长因子联合促进许旺细胞再生的最佳比例和浓度，其结果为22.86ng/mL神经生长因子和4.29ng/mL碱性成纤维生长因子的组合促进大鼠原代许旺细胞增殖的效果最好。基于该浓度组合，利用水/油/水复乳溶剂挥发法成功制备了神经生长因子-碱性成纤维生长因子-聚丙交酯-共-乙交酯缓释微球，并以其联合小间隙套管桥接技术修复SD大鼠坐骨神经横断损伤，结果显示这种微球可在结构和功能层面有效修复周围神经损伤，且效果优于神经外膜缝合、小间隙套管桥接以及无药微球联合小间隙套管桥接修复。

https://orcid.org/0000-0003-1201-7216 (Yan-Hua Wang)

关键词: 周围神经损伤, 缓释微球, 仿生微流控芯片, 小间隙套管桥接, 体外仿生微环境, 生长因子, 神经功能, 坐骨神经

Abstract: We previously prepared nerve growth factor poly-lactide co-glycolid sustained-release microspheres to treat rat sciatic nerve injury using the small gap sleeve technique. Multiple growth factors play a synergistic role in promoting the repair of peripheral nerve injury; as a result, in this study, we added basic fibroblast growth factors to the microspheres to further promote nerve regeneration. First, in an in vitro biomimetic microenvironment, we developed and used a drug screening biomimetic microfluidic chip to screen the optimal combination of nerve growth factor/basic fibroblast growth factor to promote the regeneration of Schwann cells. We found that 22.56 ng/mL nerve growth factor combined with 4.29 ng/mL basic fibroblast growth factor exhibited optimal effects on the proliferation of primary rat Schwann cells. The successfully prepared nerve growth factor-basic fibroblast growth factor-poly-lactide-co-glycolid sustained-release microspheres were used to treat rat sciatic nerve transection injury using the small gap sleeve bridge technique. Compared with epithelium sutures and small gap sleeve bridging alone, the small gap sleeve bridging technique combined with drug-free sustained-release microspheres has a stronger effect on rat sciatic nerve transfection injury repair at the structural and functional level. 

Key words: biomimetic microfluidic chip, growth factor, in vitro biomimetic microenvironment, nerve function, peripheral nerve injury, sciatic nerve, small gap sleeve bridging, sustained-release microspheres