中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (9): 1956-1960.doi: 10.4103/1673-5374.367974

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

血清反应因子可促进脊髓横断后的轴突再生

  

  • 出版日期:2023-09-15 发布日期:2023-03-06
  • 基金资助:
    国家自然科学基金项目(81870985),山东省高等教育科技计划项目(J18KA258),滨州医科大学徐荣祥再生医学科技计划项目(BY2020XRX06),山东省自然科学基金项目(BS2015SW021)

Serum response factor promotes axon regeneration following spinal cord transection injury

Guo-Ying Feng1, #, Nai-Li Zhang1, #, Xiao-Wei Liu1, #, Ling-Xi Tong1, Chun-Lei Zhang1, Shuai Zhou1, Lu-Ping Zhang1, Fei Huang1, 2, *   

  1. 1Institute of Neurobiology, Binzhou Medical University, Laishan, Shandong Province, China; 2School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao, Shandong Province, China
  • Online:2023-09-15 Published:2023-03-06
  • Contact: Fei Huang, PhD, hfei22518@163.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81870985 (to FH); Project of Shandong Province Higher Education Science and Technology Program, No. J18KA258 (to NLZ), Xu Rongxiang Regenerative Medicine Science and Technology Program of Binzhou Medical University, No. BY2020XRX06 (to NLZ); and the Natural Science Foundation of Shandong Province, No. BS2015SW021 (to NLZ).

摘要:

有研究发现,血清反应因子有助于周围神经的轴突再生,但其在中枢神经损伤后的作用仍不明确。为此,实验构建了T9-T10脊髓横断大鼠模型,发现损伤脊髓灰质神经元中血清反应因子的表达随着时间的延长而逐渐上调,第7天时达到峰值,随后逐渐下降。为进一步了解血清反应因子的作用,使用慢病毒载体过表达和沉默脊髓组织中血清反应因子,发现血清反应因子过表达可促进脊髓损伤大鼠运动功能恢复,生物素葡聚糖胺顺行示踪可见血清反应因子过表达可增加损伤脊髓中新生神经纤维的数量,同时透射电镜可见下轴突和髓鞘明显恢复,且沉默血清反应因子则表现出相反的迹象。提示血清反应因子在脊髓损伤后运动功能的恢复中发挥了至关重要的作用,其潜在机制可能与轴突再生的调节有关。

https://orcid.org/0000-0002-2298-4720 (Fei Huang)

关键词: 血清反应因子, 脊髓, 神经元, 脊髓横断, 运动功能, 轴突, 髓鞘, 再生, 生长相关蛋白43

Abstract: Studies have shown that serum response factor is beneficial for axonal regeneration of peripheral nerves. However, its role after central nervous system injury remains unclear. In this study, we established a rat model of T9–T10 spinal cord transection injury. We found that the expression of serum response factor in injured spinal cord gray matter neurons gradually increased with time, reached its peak on the 7th day, and then gradually decreased. To investigate the role of serum response factor, we used lentivirus vectors to overexpress and silence serum response factor in spinal cord tissue. We found that overexpression of serum response factor promoted motor function recovery in rats with spinal cord injury. Qualitative observation of biotinylated dextran amine anterograde tracing showed that overexpression of serum response factor increased nerve fibers in the injured spinal cord. Additionally, transmission electron microscopy showed that axon and myelin sheath morphology was restored. Silencing serum response factor had the opposite effects of overexpression. These findings suggest that serum response factor plays a role in the recovery of motor function after spinal cord injury. The underlying mechanism may be related to the regulation of axonal regeneration. 

Key words: axon, growth associated protein 43, motor function, myelin sheath, neuron, regeneration, serum response factor, spinal cord, spinal cord transection