中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (9): 1884-1889.doi: 10.4103/1673-5374.367983

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

脑类器官可作为神经系统疾病药物筛选的新工具

  

  • 出版日期:2023-09-15 发布日期:2023-03-06
  • 基金资助:
    国家自然科学基金项目(32000498),浙江大学城市学院启动资金项目(21000-581849),国家大学生创新创业培训计划项目(202113021024)

Brain organoids are new tool for drug screening of neurological diseases

Jin-Qi Zhou1, 2, #, Ling-Hui Zeng1, 2, #, Chen-Tao Li1, 2, Da-Hong He1, 2, Hao-Duo Zhao1, 2, Yan-Nan Xu1, 2, Zi-Tian Jin1, 2, Chong Gao1, 2, *   

  1. 1School of Medicine, Institute of Brain and Cognitive Science, Hangzhou City University, Hangzhou, Zhejiang Province, China; 2Key Laboratory of Novel Targets and Drug Study for Neural Repair of Zhejiang Province, School of Medicine, Hangzhou City University, Hangzhou, Zhejiang Province, China
  • Online:2023-09-15 Published:2023-03-06
  • Contact: Chong Gao, PhD, gaoc@zucc.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 32000498; the Startup Funding of Zhejiang University City College, No. 210000-581849 (both to CG); and National College Students’ Innovative Entrepreneurial Training Plan Program, No. 202113021024 (to JQZ). 

摘要:

在体外药物筛选层面,以高内涵系统为核心的表型分析系统的发展与成熟为高通量药物筛选提供了强有力的平台支持。脑类器官作为新型体外三维疾病模型,其组内质量控制、关键表征指纹以及药物筛选方案,尤其是海量中药单体筛选策略的制订等问题,目前尚缺乏系统性报道。此次综述回顾了脑类器官的发展及其相对于诱导性神经元或细胞系在疾病模拟层面的优势,同时还根据脑类器官本身的特征结合当前高内涵技术的应用与发展,从组内质量控制、类器官的诱导的标准化以及筛选方案等层面进行了展望。

https://orcid.org/0000-0001-7639-0808 (Chong Gao)

关键词: 脑类器官, 疾病模型, 高内涵技术, 多组学分析, 网络药理学, 神经变性, 表型指纹, 精神疾病, 干细胞, 中药药物筛选

Abstract: At the level of in vitro drug screening, the development of a phenotypic analysis system with high-content screening at the core provides a strong platform to support high-throughput drug screening. There are few systematic reports on brain organoids, as a new three-dimensional in vitro model, in terms of model stability, key phenotypic fingerprint, and drug screening schemes, and particularly regarding the development of screening strategies for massive numbers of traditional Chinese medicine monomers. This paper reviews the development of brain organoids and the advantages of brain organoids over induced neurons or cells in simulated diseases. The paper also highlights the prospects from model stability, induction criteria of brain organoids, and the screening schemes of brain organoids based on the characteristics of brain organoids and the application and development of a high-content screening system. 

Key words: brain organoids, disease modeling, high-content system, multiple omic analysis, network pharmacology, neurodegeneration, phenotypic fingerprint, psychiatric diseases, stem cells, traditional Chinese medicine drug screening