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    Diffusion tensor imaging reveals brain structure changes in dogs after spinal cord injury
  • Figure 7|Immunohistochemical staining of the CP in a dog model of SCI at 12 weeks after injury.

    To explore the possible mechanisms underlying the DTI alterations in the ROIs, immunohistochemical staining was performed on the four regions to observe whether the axons, glial cells, or neurons changed after SCI. The NF staining intensity for CP remained low, and the percentage of positive staining was significantly decreased at 12 weeks after injury compared with that pre-SCI (P = 0.002), as measured by quantitative analysis (Figure 7). 


    Figure 8|Immunohistochemical staining of the PLIC in a dog model of SCI at 12 weeks after injury.

    The GFAP staining intensity was more prevalent, and the percentage of positive staining was increased significantly compared with that pre-SCI (P = 0.010). The NeuN staining intensity showed no significant changes compared with that pre-SCI (P = 0.798). The NF staining for PLIC intensity remained low, and the percentage of positive staining was decreased significantly at 12 weeks after injury compared with that pre-SCI (P = 0.014), as measured by quantitative analysis (Figure 8).


    Figure 9|Immunohistochemical staining of the POG in a dog model of SCI at 12 weeks after injury.


    Figure 10|Immunohistochemical staining of the PRG in a dog model of SCI at 12 weeks after injury.

    The GFAP staining intensity was more prevalent, and the percentage of positive staining was increased significantly compared with those pre-SCI (P < 0.001). The NeuN staining intensity showed no significant change compared with that pre-SCI (P = 0.520). For POG (Figure 9), the staining intensities of both NF (P = 0.001) and GFAP (P = 0.045) were more prevalent, and the percentages of positive staining were increased significantly compared with those pre-SCI, whereas the percentage of NeuN positive staining was increased but did not reach a significant difference compared with that pre-SCI (P = 0.496). For PRG (Figure 10), the staining intensities of NF (P = 0.034), GFAP (P = 0.015) and NeuN (P = 0.036) were all more prevalent, and the percentages of positive staining were significantly increased compared with those pre-SCI. The detailed Student’s t-test results of immunohistochemical analyses comparing the SCI group with the control group are listed in Additional Table 4.


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  • 发布日期: 2022-06-17  浏览: 185
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