神经退行性病

    The Alzheimer’s disease-associated gene TREML2 modulates inflammation by regulating microglia polarization and NLRP3 inflammasome activation
  • Figure 2|Microglial TREML2 levels increase under inflammatory condition. 

    Microglia-mediated inflammation contributes to the pathogenesis of AD (Leng and Edison, 2021). Previous studies have shown that TREML2 is mainly expressed by microglia (Benitez et al., 2014; Zheng et al., 2016). Therefore, we investigated the role of TREML2 in neuroinflammation using primary microglia. As shown in Figure 2A, the population of primary microglia isolated from neonatal WT mice was over 95% pure, as indicated by double immunofluorescence staining. To mimic the neuroinflammation that occurs in the context of AD, primary microglia were stimulated with PBS or LPS (10 ng/mL, 100 ng/mL, or 1000 ng/mL) for 24 hours. As shown in Figure 2B, TREML2 levels increased in response to stimulation with 100 ng/mL or 1000 ng/mL LPS (all P < 0.05). As stimulation with 1000 ng/mL LPS markedly decreased microglia proliferation (Figure 2C; P < 0.05), an LPS dose of 100 ng/mL was used in subsequent experiments. 


    点击此处查看全文


  • 发布日期: 2022-07-20  浏览: 206
分享