神经退行性病
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Figure 6｜Decreasing Crry expression in the brain can delay neuron loss in P301S transgenic mice.
P301S mice had a lower neuron count in the hippocampus and cortex compared with WT mice. In addition, the neuron count in these two brain regions of P301S mice injected with control shRNA was significantly lower than that of P301S mice injected with Crry shRNA (both P < 0.05; Figure 6A–C). We found higher cleaved caspase-3 levels in the hippocampus and cortex of P301S mice compared with those in WT mice, as measured by western blot. Moreover, cleaved caspase-3 expression was lower in P301S mice that received Crry shRNA compared with that in P301S mice that received control shRNA (Figure 6D and E), which suggests that Crry shRNA may have reduced apoptosis. Furthermore, we analyzed hippocampal atrophy and ventricle volume based on cresyl violet staining. We observed hippocampal atrophy in P301S mice, and Crry silencing significantly alleviated hippocampal atrophy (P < 0.05; Figure 6F). Ventricle volume was increased in P301S mice, and Crry silencing decreased this change in ventricle volume (P < 0.05; Figure 6G).