脊髓损伤

    Circulating exosomal lncRNA contributes to the pathogenesis of spinal cord injury in rats
  • Figure 2|Establishment of a rat model of spinal cord injury and evaluation by functional score and hematoxylin and eosin staining. 

    The experimental group rats had significantly lower limb function scores (i.e. Tarlov’s score 1–2) after spinal cord hemisection (P < 0.001) compared with the control group, as shown in Figure 2A. This observation suggested that we successfully established the SCI rat model. In addition, HE-stained images showed that the spinal cord was injured in rats from the experimental group (Figure 2B), which was consistent with the functional finding described above.

    Figure 3|Extraction and identification of circulating exosomes. 

    To determine the features of the exosomes that were extracted from the plasma of the six experimental rats, Western blot and electron microscopy were used to detect the protein markers and the structure of exosomes, respectively. CD9 and HSP70, as two of the most classical protein markers for exosomes (Kowal et al., 2016), were chosen for detection. The representative Western blot shown in Figure 3A demonstrated that the samples from both groups were positive for CD9 and HSP70 (Figure 3A), indicating that exosomes were extracted successfully. In addition to western blotting, electron microscopy also showed that these exosomes were 62–91 nm in diameter and were circular vesicles with clear and complete membrane structures, and indicated by the arrow in Figure 3B. Measurement by NTA showed that the average exosome diameter was about 145.1 nm. Collectively, these results confirm that the extracted exosomes were suitable for use in the following analyses.


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  • 发布日期: 2022-10-31  浏览: 181
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