脊髓损伤
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Figure 3｜Identification of ADSCs.
ADSCs were isolated and characterized at passage 3 (P3) for cell transplantation after SCI. When they reached 80–90% confluence, ADSCs exhibited strong proliferative capacity and spindle-shaped morphology in a relatively homogeneous population (Figure 3A). Flow cytometry was performed to evaluate surface antigen expression, and the ADSCs stained positively for CD34, CD44, CD90, and CD105, but negatively for HLA-DR (Figure 3B–F).
Figure 8｜Administration of ADSCs decreases Iba1 expression in lamina I and II of the lumbar spinal cord dorsal horn after SCI.
Figure 9｜Administration of ADSCs decreases GFAP expression in lamina I and II of the lumbar spinal cord dorsal horn after SCI.
Previous studies have demonstrated that inflammatory changes in the spinal cord dorsal horn below the lesion site are associated with the development of NP behaviors (Li et al., 2020b; Iqubal et al., 2021). Therefore, we quantified Iba1 and GFAP intensity in the lumbar spinal dorsal horn after SCI at the T11 level (Figures 8 and 9).