脑损伤
-
Figure 2｜ NAD treatment attenuates HI in neonatal mice.
The level of cerebral injury 24 hours after HI induction was evaluated according to infarct volume. As shown in Figure 2A, TTC staining and quantitative analysis revealed that NAD doses of 5 mg/kg (P < 0.0001) and 10 mg/kg (P < 0.0001) could reduce cerebral infarction volume, and both concentrations produced similar results; therefore, intraperitoneal injection with 5 mg/kg was utilized for subsequent experiments (Figure 2A and B).
In the Sham and Sham + NAD groups, the cortical neurons (CA1 and CA3) and dentate gyrus of the hippocampus were oval or round; nuclei were completely, clearly, and neatly arranged. Lethal nuclear abnormalities, disrupted neuronal arrangements, and neuronal absence were observed in the HI group. NAD significantly reduced the extents of neuronal degeneration and necrosis (Figure 2C).