Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (22): 1995-2001.doi: 10.4103/1673-5374.145383

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CD93 and GIPC expression and localization during central nervous system inflammation

Chun Liu 1, Zhichao Cui 2, Shengjie Wang 1, Dongmei Zhang 2   

  1. 1 Experimental Animal Center, Key Laboratory of Inflammation and Molecular Drug Targets of Jiangsu Province, Nantong University, Nantong, Jiangsu Province, China
    2 Department of Pathogen Biology, Medical School of Nantong University, Nantong, Jiangsu Province, China
  • Received:2014-09-05 Online:2014-11-25 Published:2014-11-25
  • Contact: Dongmei Zhang, M.D., Department of Pathogen Biology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China, zdm@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31170766; the Nantong Municipal Social Undertakings Technological Innovation and Demonstration Project Foundation, No. HS2012032; the Natural Science Pre-research Project Foundation of Nantong University in 2012, No. 12ZY020.

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Abstract:

CD93 and GAIP-interacting protein, C termius (GIPC) have been shown to interactively alter phagocytic processes of immune cells. CD93 and GIPC expression and localization during central nervous system inflammation have not yet been reported. In this study, we established a rat model of brain inflammation by lipopolysaccharide injection to the lateral ventricle. In the brain of rats with inflammation, western blots showed increased CD93 expression that decreased over time. GIPC expression was unaltered. Immunohistochemistry demonstrated extensive distribution of CD93 expression mainly in cell membranes in the cerebral cortex. After lipopolysaccharide stimulation, CD93 expression increased and then reduced, with distinct staining in the cytoplasm and nucleus. Double immunofluorescence staining in cerebral cortex of normal rats showed that CD93 and GIPC widely expressed in resting microglia and neurons. CD93 was mainly expressed in microglial and neuronal cell membranes, while GIPC was expressed in both cell membrane and cytoplasm. In the cerebral cortex at 9 hours after model establishment, CD93-immunoreactive signal diminished in microglial membrane, with cytoplasmic translocation and aggregation detected. GIPC localization was unaltered in neurons and microglia. These results are the first to demonstrate CD93 participation in pathophysiological processes of central nervous system inflammation.

Key words: nerve regeneration, central nervous system, brain inflammation model, CD93, GIPC, neurons, microglia, expression and localization, lipopolysaccharide, intracerebroventricular injection, rats, inducible nitric oxide synthase, NSFC grants, neural regeneration