Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (10): 1612-1616.doi: 10.4103/1673-5374.167759

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Co-culture of oligodendrocytes and neurons can be used to assess drugs for axon regeneration in the central nervous system

Lin Gang1, 2, #, Yu-chen Yao1, #, Ying-fu Liu3, #, Yi-peng Li1, 2, Kai Yang1, 4, Lei Lu1, 2, Yuan-chi Cheng1, 4, Xu-yi Chen1, 5, *, Yue Tu1, 5, *   

  1. 1 Neurological Hospital of Affiliated Hospital of Logistics University of Chinese People’s Armed Police Force, Tianjin, China
    2 Tianjin University of Traditional Chinese Medicine, Tianjin, China
    3 Logistics University of Chinese People’s Armed Police Forces, Tianjin, China
    4 Liaoning Medical University, Jinzhou, Liaoning Province, China
    5 Key Laboratory of Nerve Trauma Repair, Tianjin, China
  • Received:2015-08-03 Online:2015-10-28 Published:2015-10-28
  • Contact: Yue Tu, M.D. or Xu-yi Chen, M.D.,ytumail@vip.126.com or chenxuyi1979@126.com.
  • Supported by:

    This study was supported by the Youth Program of the National Natural Science Foundation
    of China, No. 11102235; the Key Science and Technology Support Project of Tianjin City of China, No. 14ZCZDGX00500; the Key Program of the Natural Science Foundation of Tianjin City of China, No. 12JCZDJC24100; the Science and Technology Foundation of Health Bureau of Tianjin City of China, No. 2013KZ134, 2014KZ135; the Youth Program of the Natural Science Foundation of Tianjin City of China, No. 12JCQNJC07100; the Seed Foundation of Affiliated Hospital of Logistics
    University of Chinese People’s Armed Police Force, No. FYM201432.

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Abstract:

We present a novel in vitro model in which to investigate the efficacy of experimental drugs for the promotion of axon regeneration in the central nervous system. We co-cultured rat hippocampal
neurons and cerebral cortical oligodendrocytes, and tested the co-culture system using a Nogo-66 receptor antagonist peptide (NEP1–40), which promotes axonal growth. Primary cultured oligodendrocytes suppressed axonal growth in the rat hippocampus, but NEP1–40 stimulated axonal growth in the co-culture system. Our results confirm the validity of the neuron-oligodendrocyte co-culture system as an assay for the evaluation of drugs for axon regeneration
in the central nervous system.

Key words: nerve regeneration, experimental models, NEP1–40, oligodendrocytes, neurons, axon regeneration, Nogo, PC12 cells, neural regeneration