Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (11): 1814-1818.doi: 10.4103/1673-5374.170309

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Lentivirus-mediated Persephin over-expression in Parkinson’s disease rats

Xiao-feng Yin1, #, Hua-min Xu2, #, Yun-xia Jiang3, Yun-lai Zhi4, Yu-xiu Liu5, Heng-wei Xiang6, Kai Liu6, Xiao-dong Ding6, *, Peng Sun6, *   

  1. 1 Department of Neurosurgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
    2 Department of Physiology, Qingdao University, Qingdao, Shandong Province, China
    3 Nursing College of Qingdao University, Qingdao, Shandong Province, China
    4 Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    5 Department of Nursing, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    6 Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Received:2015-09-14 Online:2015-12-07 Published:2015-12-07
  • Contact: Peng Sun, M.D. or Xiao-dong Ding, M.D., sunpengqd@163.com or 15154258721@163.com.
  • About author:Peng Sun, M.D. or Xiao-dong Ding, M.D., sunpengqd@163.com or 15154258721@163.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81171208 and the Natural Science Foundation of Shandong Province of China, No. Z2008C06

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Abstract:

Persephin, together with glial cell line-derived neurotrophic factor and neurturin, has a neurotrophic
effect and promotes the survival of motor neurons cultured in vitro. In this study, dopaminergic neurons in the substantia nigra of rats were transfected with the Persephin gene. One week later 6-hydroxydopamine was injected into the anterior medial bundle to establish a Parkinson’s
disease model in the rats. Results found that the number of dopaminergic neurons in the substantia nigra increased, tyrosine hydroxylase expression was upregulated and concentrations of dopamine and its metabolites in corpus striatum were increased after pretreatment with Persephin gene. In addition, the rotating effect of the induced Parkinson’s disease rats was much less in the group pretreated with the Persephin gene. Persephin has a neuroprotective effect on the 6-hydroxydopamine-induced Parkinson’s disease through protecting dopaminergic neurons.

Key words: nerve regeneration, Persephin, lentivirus, Parkinson’s disease, dopaminergic neurons, gene therapy, over-expression, transfection, striatum, neural regeneration