Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (1): 132-136.doi: 10.4103/1673-5374.175059

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Spatiotemporal expression of Nogo-66 receptor after focal cerebral ischemia

Yue Cao 1, Ya-xian Dong 2, Jie Xu 3, Guo-liang Chu 3, Zhi-hua Yang 2, Yan-ming Liu 4   

  1. 1 Department of Medical Technology, Medical College of Shaoguan University, Shaoguan, Guangdong Province, China
    2 Department of Neurology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong Province, China
    3 Department of Human Anatomy, Sun Yat-Sen University, Guangzhou, Guangdong Province, China
    4 Department of Clinical Laboratory, Yue Bei People’s Hospital, Shaoguan, Guangdong Province, China
  • Received:2015-06-08 Online:2016-01-15 Published:2016-01-15
  • Contact: Jie Xu, xujie@gzsums.edu.cn.
  • Supported by:

    This study was supported by grants of the Guangdong Provincial Science and Technology Project in China, No. 2013B022000098 and the Scientific Research Project of Guangzhou Medical University in China in 2014, No. 2014C26.

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Abstract:

NgR, the receptor for the neurite outgrowth inhibitor Nogo-66, plays a critical role in the plasticity and regeneration of the nervous system after injury such as ischemic stroke. In the present study, we used immunohistochemistry to investigate the regional expression of NgR in rat brain following middle cerebral artery occlusion (MCAO). NgR protein expression was not observed in the center of the lesion, but was elevated in the marginal zone compared with control and sham-operated rats. The cerebral cortex and hippocampus (CA1, CA2, and CA3) showed the greatest expression of NgR. Furthermore, NgR expression was higher in the ipsilesional hemisphere than on the control side in the same coronal section. Although time-dependent changes in NgR expression across brain regions had their own characteristics, the overall trend complied with the following rules: NgR expression changes with time showed two peaks and one trough; the first peak in expression appeared between 1 and 3 days after MCAO; expression declined at 5 days; and the second peak occurred at 28 days.

Key words: nerve regeneration, focal cerebral ischemia, cerebral cortex, hippocampus, NgR, Nogo-A, immunohistochemistry, neural regeneration