Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (2): 270-277.doi: 10.4103/1673-5374.177735

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Neuroprotection of Chrysanthemum indicum Linne against cerebral ischemia/reperfusion injury by anti-inflammatory effect in gerbils

Ki-Yeon Yoo 1, In Hye Kim 2, Jeong-Hwi Cho 2, Ji Hyeon Ahn 2, Joon Ha Park 2, Jae-Chul Lee 2, Hyun-Jin Tae 3, Dae Won Kim 4,Jong-Dai Kim 5, Seongkweon Hong 6, Moo-Ho Won 2, Il Jun Kang 7   

  1. 1 Department of Oral Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Republic of Korea
    2 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea
    3 Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Republic of Korea
    4 Department of Biochemistry and Molecular Biology, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Republic of Korea
    5 Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea
    6 Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea
    7 Department of Food Science and Nutrition, Hallym University, Chuncheon, Republic of Korea
  • Received:2015-09-28 Online:2016-02-15 Published:2016-02-15
  • Contact: Moo-Ho Won, DVM, Ph.D. or Il-Jun Kang, Ph.D.,mhwon@kangwon.ac.k or ijkang@hallym.ac.kr.
  • Supported by:

    This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2013R1A2A2A01068190), and by Hallym University Specialization Fund (HRF-S-13).

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Abstract:

In this study, we tried to verify the neuroprotective effect of Chrysanthemum indicum Linne (CIL) extract, which has been used as a botanical drug in East Asia, against ischemic damage and to explore the underlying mechanism involving the anti-inflammatory approach. A gerbil was given CIL extract for 7 consecutive days followed by bilateral carotid artery occlusion to make a cerebral ischemia/reperfusion model. Then, we found that CIL extracts protected pyramidal neurons in the hippocampal CA1 region (CA1) from ischemic damage using neuronal nucleus immunohistochemistry and Fluoro-Jade B histofluorescence. Accordingly, interleukin-13 immunoreactivities in the CA1 pyramidal neurons of CIL-pretreated animals were maintained or increased after cerebral ischemia/reperfusion. These findings indicate that the pre-treatment of CIL can attenuate neuronal damage/death in the brain after cerebral ischemia/reperfusion via an anti-inflammatory
approach.

Key words: nerve regeneration, transient cerebral ischemia, delayed neuronal death, pyramidal neurons, inflammatory cytokines, neural regeneration