Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (6): 983-987.doi: 10.4103/1673-5374.184499

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ROCK inhibition enhances neurite outgrowth in neural stem cells by upregulating YAP expression in vitro

Xu-feng Jia1, #, Fei Ye2, #, Yan-bo Wang2, Da-xiong Feng2, *   

  1. 1 Department of Orthopedic Surgery, Jianyang People’s Hospital of Sichuan Province, Jianyang, Sichuan Province, China 2 Department of Spine Surgery, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan Province, China
  • Received:2016-04-28 Online:2016-06-30 Published:2016-06-30
  • Contact: Da-xiong Feng, M.D., fdxlz2002@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (General Program), No. 30872602.

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Abstract:

Spontaneous axonal regeneration of neurons does not occur after spinal cord injury because of inhibition by myelin and other inhibitory factors. Studies have demonstrated that blocking the Rho/Rho-kinase (ROCK) pathway can promote neurite outgrowth in spinal cord injury models. In the present study, we investigated neurite outgrowth and neuronal differentiation in neural stem cells from the mouse subventricular zone after inhibition of ROCK in vitro. Inhibition of ROCK with Y-27632 increased neurite length, enhanced neuronal differentiation, and upregulated the expression of two major signaling pathway effectors, phospho-Akt and phospho-mitogen-activated protein kinase, and the Hippo pathway effector YAP. These results suggest that inhibition of ROCK mediates neurite outgrowth in neural stem cells by activating the Hippo signaling pathway.

Key words: nerve regeneration, spinal cord injury, neural stem cells, Rho signaling pathway, neurite outgrowth, myelin, YAP, neural regeneration