Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (6): 1001-1005.doi: 10.4103/1673-5374.184504

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miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1

Tian-mei Qian#, Li-li Zhao#, Jing Wang, Ping Li, Jing Qin, Yi-sheng Liu, Bin Yu, Fei Ding, Xiao-song Gu, Song-lin Zhou*   

  1. Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Co-innovation Center of Neuroregeneration, Nantong, Jiangsu Province, China
  • Received:2015-12-22 Online:2016-06-30 Published:2016-06-30
  • Contact: Song-lin Zhou, Ph.D., songlin.zhou@ntu.edu.cn.
  • Supported by:

    This study was supported by the National Key Basic Research Program of China, No. 2014CB542202; the National High-Tech R&D Program of China (863 Program), No. 2012AA020502; the National Natural Science Foundation of China, No. 81130080, 81371389 and 81571198; the Natural Science Foundation of Nantong University of China, No. 13040397; the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Abstract:

MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro.

Key words: nerve regeneration, sciatic nerve injury, miR-148b-3p, Schwann cells, migration, Cand1, gene expression, microarray, peripheral nerve injury, mechanisms, neural regeneration