Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (1): 31-38.doi: 10.3969/j.issn.1673-5374.2013.01.004

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Multi-porous electroactive poly(L-lactic acid)/ polypyrrole composite micro/nano fibrous scaffolds promote neurite outgrowth in PC12 cells

Qiaozhen Yu1, Shuiling Xu2, Kuihua Zhang1, Yongming Shan3   

  1. 1 College of Materials and Textile Engineering, Jiaxing Key Laboratory of Modern Textile and Garment, Jiaxing University, Jiaxing 314001, Zhejiang Province, China
    2 College of Medicine, Jiaxing University, Jiaxing 314001, Zhejiang Province, China
    3 College of Mathematics Physics and Information Engineering, Jiaxing University, Jiaxing 314001, Zhejiang Province, China
  • Online:2013-01-05 Published:2013-01-05
  • Contact: Qiaozhen Yu, College of Materials and Textile Engineering, Jiaxing Key Laboratory of Modern Textile and Garment, Jiaxing University, Jiaxing 314001, Zhejiang Province, China,w2003yqz@126.com.
  • About author:Qiaozhen Yu☆, M.D., Associate professor.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 51073072, the Natural Science Foundation of Zhejiang Province in China, No. Y4100745, the Key Laboratory Open Foundation of Advanced Textile Materials & Manufacturing Technology of Zhejiang Sci-Tech University from Ministry of Education of China, No. 2009007, and the Science and Technology Commission of Jiaxing Municipality Program, No. 2010AY1089.

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Abstract:

In this study, poly(L-lactic acid)/ammonium persulfate doped-polypyrrole composite fibrous scaffolds with moderate conductivity were produced by combining electrospinning with in situ polymerization. PC12 cells were cultured on these fibrous scaffolds and their growth following electrical stimulation (0–20.0 µA stimulus intensity, for 1–4 days) was observed using inverted light microscopy, and scanning electron microscopy coupled with the MTT cell viability test. The results demonstrated that the poly(L-lactic acid)/ammonium persulfate doped-polypyrrole fibrous scaffold was a dual multi-porous micro/nano fibrous scaffold. An electrical stimulation with a current intensity 5.0–   10.0 µA for about 2 days enhanced neuronal growth and neurite outgrowth, while a high current intensity (over 15.0 µA) suppressed them. These results indicate that electrical stimulation with a moderate current intensity for an optimum time frame can promote neuronal growth and neurite outgrowth in an intensity- and time-dependent manner.

Key words: neural regeneration, tissue engineering, poly(L-lactic acid)/polypyrrole composite, multi-porous fibrous scaffold, electrical stimulation, PC12 cell lines, axon, electric spinning, grants-supported paper, photographs-containing paper, neuroregeneration