Neural Regeneration Research ›› 2017, Vol. 12 ›› Issue (1): 118-124.doi: 10.4103/1673-5374.198997

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Correlation between receptor-interacting protein 140 expression and directed differentiation of human embryonic stem cells into neural stem cells

Zhu-ran Zhao1, Wei-dong Yu2, Cheng Shi3, Rong Liang3, Xi Chen3, Xiao Feng4, Xue Zhang1, Qing Mu1, Huan Shen3, Jing-zhu Guo1   

  1. 1 Department of Pediatrics, Peking University People’s Hospital, Beijing, China; 
    2 Institute of Clinical Molecular Biology, Peking University People’s Hospital, Beijing, China; 
    3 Department of Obstetrics and Gynecology, Peking University People’s Hospital, Beijing, China; 
    4 Department of Pediatrics, Peking University International Hospital, Beijing, China
  • Received:2016-12-10 Online:2017-01-15 Published:2017-01-15
  • Contact: Jing-zhu Guo, Ph.D., gjzhxx@sina.com.cn.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31340024.

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Abstract:

Overexpression of receptor-interacting protein 140 (RIP140) promotes neuronal differentiation of N2a cells via extracellular regulated kinase 1/2 (ERK1/2) signaling. However, involvement of RIP140 in human neural differentiation remains unclear. We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells. Moreover, RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation, and positively correlated with the neural stem cell marker Nestin during later stages. Thus, ERK1/2 signaling may provide the underlying molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.

Key words: nerve regeneration, receptor-interacting protein 140, neural stem cells, human embryonic stem cells, directed differentiation, Oct4, Sox2, Nestin, extracellular regulated kinase 1/2 signaling pathway, neural regeneration