Abstract: The early developmental period is a critical window during which brain cells mature and contribute to both brain development and later life functions. Gamma-aminobutyric acid (GABA), recognized as a major neurotransmitter, plays a crucial role in coordinating synapse formation, neuronal proliferation, and migration during this time. Notably, recent studies have distinguished two modes of GABA action in the brain: a fast, phasic mode mediated by synaptic GABA release from GABAergic neurons, and a slower, tonic mode resulting from GABA release by non-neuronal cells, particularly astrocytes, which primarily inhibits neuronal activity and synaptic transmission, regulating various cognitive functions (Koh et al., 2023). However, the specific role of tonic GABA effects during the developmental period remains underexplored (Figure 1A). Here, we aim to examine the potential role of astrocytes in regulating GABA tone during development, thereby stimulating further investigation into GABA tone during this critical period.