Abstract:

Astrocytes have important neurosupportive functions in the brain that are altered in neurodegenerative diseases by unresolved mechanisms. We showed previously that astrocytes cultured from mice transgenic for human P301S-tau (P301S-mice) recapitulate the deficit in production and secretion of thrombospondin1 found in symptomatic P301S mouse brains, causing both reduced synapse formation and survival of cultured neurons. To further characterize how P301S-derived astrocytes differ from controls, we have compared the astrocyte-conditioned media of cultured astrocytes from postnatal day 7/8 P301S mice (P301S-astrocyte-conditioned media) versus controls (C57-astrocyte-conditioned media) using label-free liquid chromatography-mass spectrometry. We verified that thrombospondin1 secretion was significantly reduced in the P301S-astrocyte-conditioned media versus C57-astrocyte-conditioned media, demonstrating the robustness of the analysis. The most notable distinction was that ~57% of the P301S-astrocyte-conditioned media-enriched proteins were cytoplasmic proteins linked to cellular metabolism that are not predicted to be secreted via classical or non-classical secretion pathways, whereas ~88% of C57-astrocyte-conditioned media-enriched proteins comprised classically secreted proteins enriched in extracellular matrix components. These differences are associated with the finding that P301S-derived cultured astrocytes were smaller and in vivo appeared less mature in the cortex of P301S mice. The unconventional secretion pathway that P301S-astrocyte-conditioned media display shares similarities with several amyloid-β-exposed astrocyte-conditioned media, indicating that stimuli induced by tau and amyloid-β may induce a common adverse response pathway. Altogether, members of this adverse pathway may serve as a potential set of biomarkers to aid the clinical diagnosis of Alzheimer’s disease and other tauopathies, while the list of reduced neurosupportive factors could indicate new approaches to enhance neuronal survival by factor supplementation in tauopathies.

Key words: astrocyte conditioned medium, basal metabolism, extracellular matrix, nerve regeneration, neuronal survival, P301S tau transgenic mice, structural maturation, tau, tauopathy, unconventional secretion