Neural Regeneration Research ›› 2018, Vol. 13 ›› Issue (6): 1007-1012.doi: 10.4103/1673-5374.233443

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Enhancement of matrix metalloproteinases 2 and 9 accompanied with neurogenesis following collagen glycosaminoglycan matrix implantation after surgical brain injury

Wei-Cherng Hsu1, 2, Chun-Hsien Yu2, 3, Woon-Man Kung4, 5, Kuo-Feng Huang2, 4   

  1. 1 Department of Ophthalmology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    2 School of Medicine, Buddhist Tzu Chi University, Hualien, Taiwan, China;
    3 Department of Pediatrics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    4 Division of Neurosurgery, Department of Surgery, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan, China;
    5 Department of Exercise and Health Promotion, College of Education, Chinese Culture University, Taipei, Taiwan, China
  • Received:2018-04-02 Online:2018-06-15 Published:2018-06-15
  • Contact: Kuo-Feng Huang, M.D., Ph.D.,kuofeng1234@gmail.com.
  • Supported by:

    This study was supported by grants from the National Science Council of China (NSC 102-2314-B-303-004) and the Tzu Chi Medical Mission Project 105-06, Buddhist Tzu Chi Medical Foundation

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Abstract:

Surgical brain injury may result in irreversible neurological deficits. Our previous report showed that partial regeneration of a traumatic brain lesion is achieved by implantation of collagen glycosaminoglycan (CGM). Matrix metalloproteinases (MMPs) may play an important role in neurogenesis but there is currently a lack of studies displaying the relationship between the stimulation of MMPs and neurogenesis after collagen glycosaminoglycan implantation following surgical brain trauma. The present study was carried out to further examine the expression of MMP2 and MMP9 after implantation of collagen glycosaminoglycan (CGM) following surgical brain trauma. Using the animal model of surgically induced brain lesion,we implanted CGM into the surgical trauma. Rats were thus divided into three groups: (1) sham operation group: craniotomy only; (2) lesion (L) group: craniotomy + surgical trauma lesion; (3) lesion + CGM (L +CGM) group: CGM implanted following craniotomy and surgical trauma lesion. Cells positive for SOX2 (marker of proliferating neural progenitor cells) and matrix metalloproteinases (MMP2 and MMP9) in the lesion boundary zone were assayed and analyzed by immunofluorescence and ELISA commercial kits,respectively. Our results demonstrated that following implantation of CGM after surgical brain trauma,significant increases in MMP2+/SOX2+ cells and MMP9+/SOX2+ cells were seen within the lesion boundary zone in the L + CGM group. Tissue protein concentrations of MMP2 and MMP9 also increased after CGM scaffold implantation. These findings suggest that implantation of a CGM scaffold alone after surgical brain trauma can enhance the expression of MMP2 and MMP9 accompanied by neurogenesis.

Key words: collagen glycosaminoglycan, matrix metalloproteinases, surgical brain trauma, neurogenesis, neural regeneration