Neural Regeneration Research ›› 2019, Vol. 14 ›› Issue (4): 632-641.doi: 10.4103/1673-5374.247467

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Expression signatures of long non-coding RNA and mRNA in human traumatic brain injury

Li-Xiang Yang 1 , Li-Kun Yang 1 , Jie Zhu 1 , Jun-Hui Chen 1 , Yu-Hai Wang 1 , Kun Xiong 2   

  1. 1 Department of Neurosurgery, 101 st Hospital of People’s Liberation Army, Wuxi, Jiangsu Province, China
    2 Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan Province, China
  • Online:2019-04-15 Published:2019-04-15
  • Contact: Kun Xiong, MD, PhD, xiongkun2001@163.com; Yu-Hai Wang, MD, PhD, wangyuhai101@126.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81571939 (to KX), 81601719 (to JZ) and 81772134 (to KX); the Key Research and Development Program of Hunan Province of China, No. 2018SK2091 (to KX); the Wu Jie-Ping Medical Foundation of the Minister of Health of China, No. 320.6750.14118 (to KX); and the Teacher Research Foundation of Central South University of China, No. 2014JSJJ026 (to KX).

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Abstract:

Long non-coding RNAs (lncRNAs) play a key role in craniocerebral disease, although their expression profiles in human traumatic brain injury are still unclear. In this regard, in this study, we examined brain injury tissue from three patients of the 101st Hospital of the Peo¬ple’s Liberation Army, China (specifically, a 36-year-old male, a 52-year-old female, and a 49-year-old female), who were diagnosed with traumatic brain injury and underwent brain contusion removal surgery. Tissue surrounding the brain contusion in the three patients was used as control tissue to observe expression characteristics of lncRNAs and mRNAs in human traumatic brain injury tissue. Volcano plot filtering identified 99 lncRNAs and 63 mRNAs differentially expressed in frontotemporal tissue of the two groups (P < 0.05, fold change > 1.2). Microarray analysis showed that 43 lncRNAs were up-regulated and 56 lncRNAs were down-regulated. Meanwhile, 59 mRNAs were up-regulated and 4 mRNAs were down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed 27 signaling pathways associated with target genes and, in particular, legionellosis and influenza A signaling pathways. Subsequently, a lncRNA-gene network was generated, which showed an absolute correlation coefficient value > 0.99 for 12 lncRNA-mR¬NA pairs. Finally, quantitative real-time polymerase chain reaction confirmed different expression of the five most up-regulated mRNAs within the two groups, which was consistent with the microarray results. In summary, our results show that expression profiles of mRNAs and lncRNAs are significantly different between human traumatic brain injury tissue and surrounding tissue, providing novel insight re¬garding lncRNAs’ involvement in human traumatic brain injury. All participants provided informed consent. This research was registered in the Chinese Clinical Trial Registry (registration number: ChiCTR-TCC-13004002) and the protocol version number is 1.0.

Key words: nerve regeneration, human traumatic brain injuries, long noncoding RNA, messenger RNA, GO analysis, real-time quantitative polymerase chain reaction, biomarkers, microarray analysis, biological processes, medical informatics, neural regeneration