Neural Regeneration Research ›› 2020, Vol. 15 ›› Issue (9): 1662-1670.doi: 10.4103/1673-5374.276359

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Offspring of rats with cerebral hypoxia-ischemia manifest cognitive dysfunction in learning and memory abilities

Lu-Lu Xue1, 3, #, Fang Wang4, #, Rui-Ze Niu5 , Ya-Xin Tan3 , Jia Liu5 , Yuan Jin3 , Zheng Ma3 , Zi-Bin Zhang6 , Ya Jiang5 , Li Chen6 , Qing-Jie Xia6 , Jun-Jie Chen7 , Ting-Hua Wang3, 6, * , Liu-Lin Xiong1, 2, *   

  1. 1 Department of Anesthesiology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China 2 School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, Australia 3 Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China 4 Department of Science and Technology, Kunming Medical University, Kunming, Yunnan Province, China 5 Department of Laboratory Zoology, Kunming Medical University, Kunming, Yunnan Province, China 6 Institute of Neurological Disease, Department of Anesthesiology, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China 7 Southwest Medical University, Luzhou, Sichuan Province, China
  • Online:2020-09-08 Published:2020-09-23
  • Contact: Liu-Lin Xiong, MD, 499465010@qq.com; Ting-Hua Wang, wangtinghua@vip.163.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81560215 (FW); the Innovative Research Team Program of Science and Technology in Yunnan Province of China, No. 2017HC007.

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Abstract: Neonatal hypoxic-ischemic encephalopathy is a serious neurological disease, often resulting in long-term neurodevelopmental disorders among surviving children. However, whether these neurodevelopmental issues can be passed to offspring remains unclear. The right common carotid artery of 7-day-old parental-generation rats was subjected to permanent ligation using a vessel electrocoagulator. Neonatal hypoxic-ischemic rat models were established by subjecting the rats to 8% O2–92% N2 for 2 hours. The results showed that 24 hours after hypoxia and ischemia, pathological damage, cerebral atrophy, liquefaction, and impairment were found, and Zea-Longa scores were significantly increased. The parental-generation rats were propagated at 3 months old, and offspring were obtained. No changes in the overall brain structures of these offspring rats were identified by magnetic resonance imaging. However, the escape latency was longer and the number of platform crossings was reduced among these offspring compared with normal rats. These results indicated that the offspring of hypoxic-ischemic encephalopathy model rats displayed cognitive impairments in learning and memory. This study was approved by the Animal Care & Welfare Committee of Kunming Medical University, China in 2018 (approval No. kmmu2019072).

Key words: cerebral atrophy, cerebral infarct, cerebral liquefaction, cognitive impairment, magnetic resonance imaging, neonatal brain hypoxia and ischemia, neuronal apoptosis, offspring