Abstract: Formation of the node of Ranvier as a highly coordinated event: Saltatory conduction ensures that information in the vertebrate nervous system is rapidly transmitted over large distances and efficiently processed in complex networks. It requires the insulation of axonal segments by myelin and the formation of highly structured nodes of Ranvier that are interspersed at regular intervals between successive myelin sheaths and regenerate the action potential as it propagates along the nerve (Rasband and Peles, 2021). To be functional, nodes of Ranvier and the adjacent paranodal and juxtaparanodal regions (jointly referred to as nodal complex) contain ordered arrays of voltage-gated ion channels. The nodal complex additionally contains a host of adhesion molecules that are either supplied by the neuron or by the axon-contacting glial cells. Formation, organization, structural stability, and maintenance of the node depend on multiple fine-tuned molecular interactions among these neuronal and glial adhesion molecules (Faivre-Sarrailh and Devaux, 2013; Rasband and Peles, 2021).