Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (22): 2060-2068.doi: 10.3969/j.issn.1673-5374.2013.22.005

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Shuyusan-containing serum protects SH-SY5Y cells against corticosterone-induced impairment

Liping Chen1, Zhigao Sun2, Fawei Wang1, Chengyong Xu2, Miao Geng3, Hongyan Chen3, Dongmei Duan1   

  1. 1 Department of Traditional Chinese Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China
    2 Department of Internal Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China
    3 Institute of Geriatrics, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2012-10-15 Revised:2013-03-25 Online:2013-08-05 Published:2013-08-05
  • Contact: Liping Chen, M.D., Chief physician, Master’s supervisor, Department of Traditional Chinese Medicine, Hainan Branch of Chinese PLA General Hospital, Sanya 572004, Hainan Province, China, lipingschen@yahoo.com.cn.

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Abstract:

The Chinese herb Shuyusan, whose main constituent is jasminoidin, has been shown to protect SH-SY5Y cells against corticosterone-induced damage. SH-SY5Y cells injured by 400 μmol/L corticosterone were treated with 5 and 30 μg/mL Shuyusan-containing serum. Results revealed that Shuyusan-containing serum elevated the survival rate of SH-SY5Y cells, reduced Bax expression, increased Bcl-2 expression, markedly elevated brain-derived neurotrophic factor mRNA expression, and blocked cell apoptosis. Moreover, the effect of high-dose (30 μg/mL) Shuyusan-containing se-rum was more remarkable. Therefore, Shuyusan-containing serum appears to protect SH-SY5Y cells against corticosterone-induced impairment by adjusting the expression of apoptosis-associ- ated proteins and brain-derived neurotrophic factor. Moreover, high-dose Shuyusan-containing se-rum has a protective effect on high-dose corticosterone-induced impairment.