Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (22): 2078-2085.doi: 10.3969/j.issn.1673-5374.2013.22.007

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Neuronal-like cell differentiation of non-adherent bone marrow cell-derived mesenchymal stem cells

Yuxin Wu1, Jinghan Zhang2, Xiaoming Ben3   

  1. 1 Department of Neurosurgery, Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
    2 Department of Newborn Medicine, Nanjing Children’s Hospital of Nanjing Medical University, Nanjing 210008, Jiangsu Province, China
    3 Department of Newborn Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, China
  • Received:2013-04-28 Revised:2013-06-30 Online:2013-08-05 Published:2013-08-05
  • Contact: Xiaoming Ben, Ph.D., Professor, Department of Newborn Medicine, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, China, benxm@163.com.
  • About author:Yuxin Wu, Associate chief physician. Yuxin Wu and Jinghan Zhang contributed equally to this work.
  • Supported by:

    国家自然科学基金(30471836)

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Abstract:

Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa-rated and cultured using the “pour-off” method. Non-adherent bone marrow cell-derived mesenchymal stem cells developed colony-forming unit-fibroblasts, and could be expanded by supplementation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth fac-tor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from β-galactosidase transgenic mice were also transplanted into focal ischemic brain (right corpus striatum) of C57BL/6J mice. At 8 weeks, cells positive for LacZ and β-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both β-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.

Key words: neural regeneration, stem cells, non-adherent bone marrow cell-derived mesenchymal stem cells, neuronal-like cells, colony-forming unit-fibroblasts, proliferation, differentiation, beta-galactosidase transgenic mouse, cell transplantation, cerebral ischemia, bone marrow cells-derived mesenchymal stem cells, grants-supported paper, neuroregeneration