Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (9): 978-985.doi: 10.4103/1673-5374.133151

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Sequential expression of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor in rat hippocampal neurons after fluid percussion injury

Zhiqiang Li 1, Qingming Shu 2, Lingzhi Li 3, Maolin Ge 4, Yongliang Zhang 5   

  1. 1 Second Department of Medicine, Inner Mongolia Corps Hospital, Chinese People’s Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China
    2 Department of Pathology, General Hospital of Chinese People’s Armed Police Forces, Beijing, China
    3 Section of Pharmaceutical Chemistry, Department of Rescue Medicine, Logistics University of Chinese People’s Armed Police Force, Tianjin, China
    4 Second Department of Surgery, Inner Mongolia Corps Hospital, Chinese People’s Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China
    5 Training Department, Logistics University of Chinese People’s Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin, China 
  • Received:2014-03-10 Online:2014-05-20 Published:2014-05-20
  • Contact: Yongliang Zhang, M.D., Training Department, Logistics University of Chinese People’s Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin 300300, China, zhang78127@tom.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30471934.

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Abstract:

Traumatic brain injury causes gene expression changes in different brain regions. Occurrence and development of traumatic brain injury are closely related, involving expression of three factors, namely cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. However, little is known about the correlation of these three factors and brain neuronal injury. In this study, primary cultured rat hippocampal neurons were subjected to fluid percussion injury according to Scott’s method, with some modifications. RT-PCR and semi-quantitative immunocytochemical staining was used to measure the expression levels of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. Our results found that cyclooxygenase-2 expression were firstly increased post-injury, and then decreased. Both mRNA and protein expression levels reached peaks at 8 and 12 hours post-injury, respectively. Similar sequential changes in glutamate receptor 2 were observed, with highest levels mRNA and protein expression at 8 and 12 hours post-injury respectively. On the contrary, the expressions of platelet activating factor receptor were firstly decreased post-injury, and then increased. Both mRNA and protein expression levels reached the lowest levels at 8 and 12 hours post-injury, respectively. Totally, our findings suggest that these three factors are involved in occurrence and development of hippocampal neuronal injury.