Brilliant blue G attenuates lipopolysaccharide- mediated microglial activation and inflammation

doi:10.3969/j.issn.1673-5374.2013.07.003

Abstract

Abstract:

Previous studies have confirmed that oxidized adenosine triphosphate, a P2X7 receptor antagonist, attenuates lipopolysaccharide-mediated microglial activation and inflammatory expression following neuronal damage in rat brain. NaCl and temperature may affect the potency of oxidized adenosine triphosphate. Brilliant blue G is a derivative of a widely used food additive and has little toxicity. This study explored the effects of brilliant blue G, a selective P2X7 receptor antagonist, on microglial activation and inflammation. Results demonstrated that brilliant blue G inhibited the release of cyclooxygenase-2 and interleukin-6 in BV2 cells. Immunofluorescence displayed that brilliant blue G could suppress lipopolysaccharide-induced microglial activation. This study used RNA interference to block P2X7 receptor expression and found that small interfering RNA also suppressed the release of cyclooxygenase-2 and interleukin-6 in BV2 cells. These results suggested that downregulation of the P2X7 receptor by brilliant blue G was involved in the inhibition of microglial activation and inflammation.

Key words: neural regeneration, neurodegenerative disease, brilliant blue G, P2X7 receptor, lipopolysaccharide, microglia, inflammatory cytokines, RNA interference, cyclooxygenase-2, interleukin-6, grants-supported paper, photographs-containing paper, neuroregeneration

Kui Lu, Jue Wang, Bin Hu, Xiaolei Shi, Junyi Zhou, Yamei Tang, Ying Peng. Brilliant blue G attenuates lipopolysaccharide- mediated microglial activation and inflammation[J]. Neural Regeneration Research, 2013, 8(7): 599-608.

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