Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (10): 869-881.doi: 10.3969/j.issn.1673-5374.2013.10.001

    Next Articles

Thrombospondin 1 promotes synaptic formation in bone marrow-derived neuron-like cells

Yun Huang, Mingnan Lu, Weitao Guo, Rong Zeng, Bin Wang, Huaibo Wang   

  1. Department of Orthopedics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
  • Received:2012-12-01 Revised:2013-03-07 Online:2013-04-05 Published:2013-04-05
  • Contact: Weitao Guo, Ph.D., Chief physician, Department of Orthopedics, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China, guoweitao2000@sina.com.
  • About author:Yun Huang★, Studying for master’s degree.
  • Supported by:
    广东省自然科学基金项目(No.S2011010004096)；广东省医学科研基金资助项目(No.A2009477，A2010431)。
     

PDF

793

Abstract:

In this study, a combination of growth factors was used to induce bone marrow mesenchymal stem cells differentiation into neuron-like cells, in a broader attempt to observe the role of thrombospondin 1 in synapse formation. Results showed that there was no significant difference in the differentiation rate of neuron-like cells between bone marrow mesenchymal stem cells with thrombospondin induction and those without. However, the cell shape was more complex and the neurites were dendritic, with unipolar, bipolar or multipolar morphologies, after induction with thrombospondin 1. The induced cells were similar in morphology to normal neurites. Immunohistochemical staining showed that the number of positive cells for postsynaptic density protein 95 and synaptophysin 1 protein was significantly increased after induction with thrombospondin 1. These findings indicate that thrombospondin 1 promotes synapse formation in neuron-like cells that are differentiated from bone marrow mesenchymal stem cells.

Key words: