Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (24): 2236-2248.doi: 10.3969/j.issn.1673-5374.2013.24.003

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Combined transplantation of GDAsBMP and hr-decorin in spinal cord contusion repair

Liang Wu1, 2, 3, Jianjun Li1, 2, Liang Chen1, 2, Hong Zhang1, Li Yuan1, 2, Stephen JA Davies4   

  1. 1 School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China
    2 Department of Neural Functional Reconstruction of Spine and Spinal Cord, China Rehabilitation Research Center, Beijing 100068, China
    3 Rehabilitation Center, Beijing Xiaotangshan Rehabilitation Hospital, Beijing 102211, China
    4 Department of Neurosurgery, University of Colorado Denver, 1250 14th Street Denver, Colorado 80217, USA
  • Received:2013-03-15 Revised:2013-06-27 Online:2013-08-25 Published:2013-08-25
  • Contact: Jianjun Li, Master, Professor, Chief physician, School of Rehabilitation Medicine, Capital Medical University, Beijing 100068, China; Department of Neural Functional Reconstruction of Spine and Spinal Cord, China Rehabilitation Research Center, Beijing 100068, China, lcrrc2007@ yahoo.com.cn; Stephen JA Davies, Ph.D., Associate professor, Department of Neurosurgery, University of Colorado Denver, 1250 14th Street Denver, Colorado 80217, USA, sdavies@bcm.edu.
  • About author:Liang Wu, Ph.D., M.D.
  • Supported by:

    中华人民共和国财政部和中国康复研究中心项目基金资助(2008-2，2008-3，2008-4，2008-5)

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Abstract:

Following spinal cord injury, astrocyte proliferation and scar formation are the main factors inhibiting the regeneration and growth of spinal cord axons. Recombinant decorin suppresses inflammatory reactions, inhibits glial scar formation, and promotes axonal growth. Rat models of T8 spinal cord contusion were created with the NYU impactor and these models were subjected to combined transplantation of bone morphogenetic protein-4-induced glial-restricted precursor-derived astro-cytes and human recombinant decorin transplantation. At 28 days after spinal cord contusion, dou-ble-immunofluorescent histochemistry revealed that combined transplantation inhibited the early in-flammatory response in injured rats. Furthermore, brain-derived neurotrophic factor, which was se-creted by transplanted cells, protected injured axons. The combined transplantation promoted ax-onal regeneration and growth of injured motor and sensory neurons by inhibiting astrocyte prolifer-ation and glial scar formation, with astrocytes forming a linear arrangement in the contused spinal cord, thus providing axonal regeneration channels.

Key words: neural regeneration, spinal cord injury, astrocytes, glial scar, neural stem cells, combined trans-plantation, glial progenitor cells, glial cells, human recombinant decorin, brain-derived growth factor, glial fibrillary acidic protein, grants-supported paper, neuroregeneration