Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (28): 2597-2604.doi: 10.3969/j.issn.1673-5374.2013.28.001

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Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

Hong Jiang1, 2, Pengfei Lv3, Jichang Li1, Hongjun Wang2, Tiezhong Zhou2, Yingzi Liu2, Wei Lin1   

  1. 1 Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China
    2 Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
    3 Scientific Research Department, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Received:2013-05-10 Revised:2013-08-02 Online:2013-10-05 Published:2013-10-05
  • Contact: Jichang Li, M.D., Professor, Institute of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China, lijichang828@sina.com. Hongjun Wang, M.D., Associate professor, Institute of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China, lnwhj2012@163.com
  • About author:Hong Jiang, Studying for doctorate, Lecturer.

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Abstract:

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate (62.5–500 μg/mL) for 24 hours resulted in PC12 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin (25, 50 and 100 μg/mL), and exposed to 125 μg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, lac-tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhib-ited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.