Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (3): 236-242.doi: 10.4103/1673-5374.128214

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660 nm red light-enhanced bone marrow mesenchymal stem cell transplantation for hypoxic-ischemic brain damage treatment

Xianchao Li1, Wensheng Hou1, 2, Xiaoying Wu1, 2, Wei Jiang3, Haiyan Chen2, Nong Xiao3, Ping Zhou2   

  1. 1 Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China
    2 Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China
    3 Rehabilitation Center, Children’s Hospital of Chongqing Medical University, Chongqing, China
  • Online:2014-02-15 Published:2014-02-15
  • Contact: Xiaoying Wu, M.D., Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China; Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing 400044, China, xiaoyingwu69@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30970758, 31271060; the National Science and Technology Support Program of China, No. 2011BAI14B04, 2012BAI16B02; the Natural Science Foundation of Chongqing in China, No. cstc2012jjA10103.

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Abstract:

Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hypoxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600–1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migration and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 × 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes the migration of bone marrow mesenchymal stem cells, thereby enhancing the contribution of cell transplantation in the treatment of hypoxic-ischemic brain damage.

Key words: nerve regeneration, stem cells, Transwell assay, red light, hypoxic-ischemic brain damage, bone marrow mesenchymal stem cells, transplantation, migration, learning ability, NSFC grant, neural regeneration