Neural Regeneration Research ›› 2014, Vol. 9 ›› Issue (8): 806-814.doi: 10.4103/1673-5374.131595

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Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury

Zhiyuan Li 1, Zhanxiu Zhang 1, Lili Zhao 1, Hui Li 1, Suxia Wang 1, Yong Shen 2   

  1. 1 Department of Joint Orthopedics, Hebei Provincial Xingtai People’s Hospital, Xingtai, Hebei Province, China
    2 Department of Spinal Orthopedics, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
  • Received:2014-02-08 Online:2014-04-25 Published:2014-04-25
  • Contact: Zhiyuan Li, Department of Joint Orthopedics, Hebei Provincial Xingtai People’s Hospital, Xingtai, Hebei Province, China, myarcher@126.com.

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Abstract:

We hypothesized that RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells before transplantation might further improve neurological function in rats with spinal cord transection injury. After 2 weeks, the number of neurons and BrdU-positive cells in the Nogo-66 receptor gene silencing group was higher than in the bone marrow mesenchymal stem cell group, and significantly greater compared with the model          group. After 4 weeks, behavioral performance was significantly enhanced in the model group. After 8 weeks, the number of horseradish peroxidase-labeled nerve fibers was higher in the Nogo-66 receptor gene silencing group than in the bone marrow mesenchymal stem cell group, and significantly higher than in the model group. The newly formed nerve fibers and myelinated nerve fibers were detectable in the central transverse plane section in the bone marrow mesenchymal stem cell group and in the Nogo-66 receptor gene silencing group.

Key words: nerve regeneration, spinal cord injury, bone marrow mesenchymal stem cells, Nogo-66 receptor, RNA interference, horseradish peroxidase, BrdU, gene silencing, neural regeneration