Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (3): 451-456.doi: 10.4103/1673-5374.153695

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Neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress in rats with streptozotocin-induced type 1 diabetes

Sang Gun Lee 1, Dae Young Yoo 2, Hyo Young Jung 2, Sung Min Nam 2, Jong Whi Kim 2, Jung Hoon Choi 3, Sun Shin Yi 4, Moo-Ho Won 5, Yeo Sung Yoon 2, In Koo Hwang 2, Seung Myung Moon 1   

  1. 1 Departments of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-907, South Korea
    2 Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National
    University, Seoul 151-742, South Korea
    3 Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea
    4 Department of Biomedical Laboratory Science, College of Medical Sciences, Soonchunhyang University, Asan 336-745, South Korea
    5 Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea
  • Received:2015-02-10 Online:2015-03-20 Published:2015-03-20
  • Contact: Seung Myung Moon, M.D., Ph.D., nsmsm@chol.com or nsmsm@hallym.ac.kr.
  • Supported by:

    This work was supported by a National Research Foundation of Korea Grant funded by the Korean Government (MEST), Republic of Korea, No. 2010-0007712.

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Abstract:

In this study, we investigated the effects of streptozotocin-induced type 1 diabetes on antioxidant-like protein-1 immunoreactivity, protein carbonyl levels, and malondialdehyde formation, a marker for lipid peroxidation, in the hippocampus. For this study, streptozotocin (75 mg/kg) was intraperitoneally injected into adult rats to induce type 1 diabetes. The three experimental parameters were determined at 2, 3, 4 weeks after streptozotocin treatment. Fasting blood glucose levels significantly increased by 20.7–21.9 mM after streptozotocin treatment. The number of antioxidant-like protein-1 immunoreactive neurons significantly decreased in the hippocampal CA1 region, but not the dentate gyrus, 3 weeks after streptozotocin treatment compared to the control group. Malondialdehyde and protein carbonyl levels, which are modified by oxidative stress, significantly increased with a peak at 3 weeks after malondialdehyde treatment, and then decreased 4 weeks after malondialdehyde treatment. These results suggest that neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress 3 weeks after malondialdehyde treatment.

Key words: nerve regeneration, hippocampus, dentate gyrus, lipid peroxidation, type 1 diabetes, malondialdehyde, neurons, neural regeneration