Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (9): 1450-1456.doi: 10.4103/1673-5374.165514

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Effects of total saponins of Panax notoginseng on immature neuroblasts in the adult olfactory bulb following global cerebral ischemia/reperfusion

Xu He1, 2, Feng-jun Deng3, Jin-wen Ge4, Xiao-xin Yan1, Ai-hua Pan1, Zhi-yuan Li1   

  1. 1 Department of Anatomy and Neurobiology, School of Basic Medical Sciences, Central South University, Changsha, Hunan Province, China
    2 Department of Anatomy, Yiyang Medical College, Yiyang, Hunan Province, China
    3 Department of Pharmacy, Yiyang Medical College, Yiyang, Hunan Province, China
    4 Department of Integrated Traditional and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province, China
  • Received:2015-08-11 Online:2015-09-28 Published:2015-09-28
  • Contact: Ai-hua Pan, Ph.D. or Zhi-yuan Li, Ph.D., panaihua@csu.edu.cn or li_zhiyuan@gibh.ac.cn.
  • Supported by:

    This study was supported by the Hunan Provincial Innovation Foundation for Postgraduate in China, No. CX2014B099 (to XH); the Science Foundation of Hunan Provincial Education Department of China, No. 11C1264 (to FJD), 13C958(to XH)

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Abstract:

The main active components extracted from Panax notoginseng are total saponins. They have been shown to inhibit platelet aggregation, increase cerebral blood flow, improve neurological behavior, decrease infarct volume and promote proliferation and differentiation of neural stem cells in the hippocampus and lateral ventricles. However, there is a lack of studies on whether total saponins of Panax notoginseng have potential benefits on immature neuroblasts in the olfactory bulb following ischemia and reperfusion. This study established a rat model of global cerebral ischemia and reperfusion using four-vessel occlusion. Rats were administered total saponins of Panax notoginseng at 75 mg/kg intraperitoneally 30 minutes after ischemia then once a day, for either 7 or 14 days. Total saponins of Panax notoginseng enhanced the number of doublecortin (DCX)+ neural progenitor cells and increased co-localization of DCX with neuronal nuclei and phosphorylated cAMP response element-binding/DCX+ neural progenitor cells in the olfactory bulb at 7 and 14 days post ischemia. These findings indicate that following global brain ischemia/reperfusion, total saponins of Panax notoginseng promote differentiation of DCX+ cells expressing immature neuroblasts in the olfactory bulb and the underlying mechanism is related to the activation of the signaling pathway of cyclic adenosine monophosphate response element binding protein.