Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (4): 682-686.doi: 10.4103/1673-5374.180758

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Complement components of nerve regeneration conditioned fluid influence the microenvironment of nerve regeneration

Guang-shuai Li1, Qing-feng Li2, *, Ming-min Dong3, *, Tao Zan2, Shuang Ding2, Lin-bo Liu1   

  1. 1 Plastic & Reconstructive Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China
    2 Plastic & Reconstructive Surgery of the Ninth People’s Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China
    3 Otolaryngology-Head & Neck Surgery of the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China
  • Received:2015-10-23 Online:2016-04-30 Published:2016-04-30
  • Contact: Qing-feng Li, M.D., or Ming-min Dong, M.D., dr.liqingfeng@shsmu.edu.cn or chnwills@hotmail.com.
  • Supported by:

    This study was supported by grants from the National Natural Science Foundation of China, No. 30925034, 81101437

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Abstract:

Nerve regeneration conditioned fluid is secreted by nerve stumps inside a nerve regeneration chamber. A better understanding of the proteinogram
of nerve regeneration conditioned fluid can provide evidence for studying the role of the microenvironment in peripheral nerve
regeneration. In this study, we used cylindrical silicone tubes as the nerve regeneration chamber model for the repair of injured rat sciatic
nerve. Isobaric tags for relative and absolute quantitation proteomics technology and western blot analysis confirmed that there were more
than 10 complement components (complement factor I, C1q-A, C1q-B, C2, C3, C4, C5, C7, C8β and complement factor D) in the nerve
regeneration conditioned fluid and each varied at different time points. These findings suggest that all these complement components have
a functional role in nerve regeneration.

Key words: nerve regeneration, peripheral nerve injury, nerve regeneration chamber model, sciatic nerve, nerve regeneration conditioned fluid, complement, iTRAQ proteomics technology, neural regeneration