Abstract: Iso?urane is a widely used inhaled anesthetic in the clinical setting. However, the mechanism underlying its effect on consciousness is under discussion. Terefore, we investigated the effect of iso?urane on the hippocampus and cortex using an in vivo feld recording approach. Our results showed that 1.3%, 0.8%, and 0.4% iso?urane exerted an inhibitory in?uence on the mouse hippocampus and cortex. Further, high frequency bands in the cortex and hippocampus showed greater suppression with increasing iso?urane concentration. Our fndings suggest that in vivo feld recordings can monitor the effect of iso?urane anesthesia on the mouse cortex and hippocampus.

Key words: nerve regeneration, neurons, iso?urane, patch clamp, cell membrane, synaptic response, inhalational anesthesia, electrophysiology